Main Menu (Mobile)- Block

Main Menu - Block

Carbon-Based Fluorogenics

janelia7_blocks-janelia7_node_tool_type_list | block
Reagents
node_title | node_title

Carbon-Based Fluorogenics

node:body | entity_field

High-contrast fluorogenic derivatives of carbofluorescein and carborhodamine

The use of fluorescence technologies for biochemi-cal and biological research has exploded in recent years. Chemical fluorophores can enable super-resolution microscopy, high-throughput screening, and functional imaging in living tissue. Despite this utility, the current collection of chemical fluoro-phores remains insufficient for advanced biological imaging experiments and other assays.

To increase the usefulness of small molecule fluorophores, researchers continue to explore new chemical scaffolds with distinct and advantageous properties. Researchers at the Howard Hughes Medical Institute’s Janelia Research Campus have synthesized carbon-containing analogs of the eminently useful xanthene-based fluorescein and rhodamine dyes that exhibit red-shifted fluores-cence properties. These green-excited “carbo-fluorescein” and “carborhodamine” dyes display excellent fluorescence brightness and their proper-ties can be tuned through structural modification. In particular, these dyes can be “masked” by attachment of different blocking groups that squelch fluorescence. Removal or modification of these groups activates fluorescence.

These fluorogenic compounds based on carbo-fluorescein and carborhodamine exhibit extremely high on/off contrast ratios, yet upon activation are extremely bright and photostable. These probes will be useful for a variety of applications, from standard biochemical assays to super-resolution microscopy.

Advantages:

  • Fluorogenic derivatives display probes have a high on/off contrast ratio, and excellent brightness
  • Similarity to xanthene dyes allows use of established masking technologies
  • Researchers have developed a strategy that allows for efficient synthesis of these probes

Applications:

  • Live cell imaging and super-resolution microscopy
  • Red-shifted fluorogenic enzyme substrates for ELISA experiments
  • Scaffolds for reaction based indicators

Opportunity:

Free for Non-Profit Research and available for Commercial License

For inquiries, please reference:

Janelia 2013-018

node:field_image | entity_field
node:field_label | entity_field
Enabling superior brightness, versatile
janelia_helper-janelia7_publication_list | block
janelia7_blocks-janelia7_tool_collaborators | block
janelia7_blocks-janelia7_program_administration | block

Contact

Michael Perham
Head of Innovation Management
addthis-addthis_block | block