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Drosophilia Genetic Tools

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Drosophilia Genetic Tools

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About the Innovation

Rubin lab Plasmid Constructs are available from Addgene, which has distributed over 430 plasmids listed below.

 

Plasmids from Pfeiffer et al. 2008 and Pfeiffer et al. 2010.

 

Name Addgene Plasmid ID 5' UTR IVS Transgene 3' UTR WPRE 3' UTR Term. Ref

pBDP

17566

 

none

 

 

Pfeiffer et al. 2008

pBPGUw

17575

 

GAL4a

 

hsp70

Pfeiffer et al. 2008

pBPGw

17574

 

GAL4a

 

hsp70

Pfeiffer et al. 2008

pBPGAL4.1Uw

26226

 

GAL4a

 

hsp70

Pfeiffer et al. 2010

pBPGAL4.2Uw-2

26227

 

GAL4a

 

SV40

Pfeiffer et al. 2010

pBPGAL4.2::VP16Uw

26228

 

GAL4::VP16a

 

hsp70

Pfeiffer et al. 2010

pBPGAL4.2::p65Uw

26229

 

GAL4::p65a

 

hsp70

Pfeiffer et al. 2010

pBPnlsLexA::GADflUw

26232

 

nlsLexA::GADfla

 

hsp70

Pfeiffer et al. 2010

pBPLexA::p65Uw

26231

 

LexA::p65a

 

hsp70

Pfeiffer et al. 2010

pBPnlsLexA::p65Uw

26230

 

nlsLexA::p65a

 

hsp70

Pfeiffer et al. 2010

pBPZpGAL4DBDUw

26233

 

Zip-GAL4DBDa

 

hsp70

Pfeiffer et al. 2010

pBPp65ADZpUw

26234

 

p65AD-Zipa

 

hsp70

Pfeiffer et al. 2010

pBPGAL80Uw-4

26235

+

GAL80a

+

hsp70

Pfeiffer et al. 2010

pBPGAL80Uw-6

26236

+

GAL80a

+

SV40

Pfeiffer et al. 2010

pJFRC-MUH

26213

 

 

 

 

Pfeiffer et al. 2010

pJFRC12-10XUAS-IVS-myr::GFP

26222

+

myr::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC14-10XUAS-IVS-GFP-WPRE

26223

+

GFP

+

SV40

Pfeiffer et al. 2010

pJFRC18-8XLexAop2-mCD8::GFP

26225

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC19-13XLexAop2-IVS-myr::GFP

26224

+

myr::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC2-10XUAS-IVS-mCD8::GFP

26214

+

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC2-INS

26215

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC3-1XUAS-IVS-mCD8::GFP

26216

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC4-3XUAS-IVS-mCD8::GFP

26217

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC5-5XUAS-IVS-mCD8::GFP

26218

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC6-15XUAS-IVS-mCD8::GFP

26219

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC7-20XUAS-IVS-mCD8::GFP

26220

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

pJFRC8-40XUAS-IVS-mCD8::GFP

26221

 

mCD8::GFP

 

SV40

Pfeiffer et al. 2010

 

BP plasmid vector backbones are derived from pBPGUw. They contain the pUC19-derived bacterial origin of replication and ampicillin resistance gene, the PhiC31 attB site, the mini-white marker for identification of transformants in Drosophila, and the DSCP basal promoter. Abbreviations: U, DSCP basal promoter; w, mini-white marker; nls, nuclear localization signal; IVS, intervening sequence within the 5’ UTR; WPRE, a Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element within the 3’ UTR; 3’ UTR Term., the transcriptional terminator; and pBP, plasmid BP backbone.

Janelia Reporter Construct (JFRC) backbones are derived from pBDP. In addition, all vectors also contain a basal promoter derived from hsp70 and an SV40 transcriptional terminator.

a = Drosophila codon-optimized transgene

 

Plasmids from Nern et al. PNAS 2011: Multiple new site-specific recombinases for use in manipulating animal genomes.

 

Name Addgene Plasmid ID

pJFRC150-20XUAS-IVS-Flp1::PEST

32132

pJFRC151-3XUAS-IVS-Flp2::PEST

32133

pJFRC153-20XUAS-IVS-B2::PEST

32134

pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP

32135

pJFRC157-20XUAS-IVS-B3::PEST

32136

pJFRC159-1XUAS-DSCP-B3::PEST

32137

pJFRC158-3XUAS-IVS-B3::PEST

32138

pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

32139

pJFRC161-20XUAS-IVS-KD::PEST

32140

pJFRC164-21XUAS-KDRT>-dSTOP-KDRT>-myr::RFP

32141

pJFRC165-20XUAS-IVS-R::PEST

32142

pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP

32143

pJFRC170-3XUAS-IVS-Cre::PEST

32144

pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

32145

pJFRC173-20XUAS-IVS-Dre::PEST

32146

pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP

32147

pBPhsFlp1

32148

pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

32149

 

Plasmids from Pfeiffer et al. PNAS 2012: Using translational enhancers to increase transgene expression in Drosophila

 

Name Addgene Plasmid ID

pJFRC28-10XUAS-IVS-GFP-p10

36431

pJFRC81-10XUAS-IVS-Syn21-GFP-p10

36432

 

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Contact

Michael Perham
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