Drosophilia Genetic Tools

Open Science

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Drosophilia Genetic Tools

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About the Innovation

Rubin lab Plasmid Constructs are available from Addgene, which has distributed over 430 plasmids listed below.

Plasmids from Pfeiffer et al. 2008 and Pfeiffer et al. 2010.

Name	Addgene Plasmid ID	5' UTR IVS	Transgene	3' UTR WPRE	3' UTR Term.	Ref
pBDP	17566		none			Pfeiffer et al. 2008
pBPGUw	17575		GAL4a		hsp70	Pfeiffer et al. 2008
pBPGw	17574		GAL4a		hsp70	Pfeiffer et al. 2008
pBPGAL4.1Uw	26226		GAL4a		hsp70	Pfeiffer et al. 2010
pBPGAL4.2Uw-2	26227		GAL4a		SV40	Pfeiffer et al. 2010
pBPGAL4.2::VP16Uw	26228		GAL4::VP16a		hsp70	Pfeiffer et al. 2010
pBPGAL4.2::p65Uw	26229		GAL4::p65a		hsp70	Pfeiffer et al. 2010
pBPnlsLexA::GADflUw	26232		nlsLexA::GADfla		hsp70	Pfeiffer et al. 2010
pBPLexA::p65Uw	26231		LexA::p65a		hsp70	Pfeiffer et al. 2010
pBPnlsLexA::p65Uw	26230		nlsLexA::p65a		hsp70	Pfeiffer et al. 2010
pBPZpGAL4DBDUw	26233		Zip-GAL4DBDa		hsp70	Pfeiffer et al. 2010
pBPp65ADZpUw	26234		p65AD-Zipa		hsp70	Pfeiffer et al. 2010
pBPGAL80Uw-4	26235	+	GAL80a	+	hsp70	Pfeiffer et al. 2010
pBPGAL80Uw-6	26236	+	GAL80a	+	SV40	Pfeiffer et al. 2010
pJFRC-MUH	26213					Pfeiffer et al. 2010
pJFRC12-10XUAS-IVS-myr::GFP	26222	+	myr::GFP		SV40	Pfeiffer et al. 2010
pJFRC14-10XUAS-IVS-GFP-WPRE	26223	+	GFP	+	SV40	Pfeiffer et al. 2010
pJFRC18-8XLexAop2-mCD8::GFP	26225		mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC19-13XLexAop2-IVS-myr::GFP	26224	+	myr::GFP		SV40	Pfeiffer et al. 2010
pJFRC2-10XUAS-IVS-mCD8::GFP	26214	+	mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC2-INS	26215		mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC3-1XUAS-IVS-mCD8::GFP	26216		mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC4-3XUAS-IVS-mCD8::GFP	26217		mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC5-5XUAS-IVS-mCD8::GFP	26218		mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC6-15XUAS-IVS-mCD8::GFP	26219		mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC7-20XUAS-IVS-mCD8::GFP	26220		mCD8::GFP		SV40	Pfeiffer et al. 2010
pJFRC8-40XUAS-IVS-mCD8::GFP	26221		mCD8::GFP		SV40	Pfeiffer et al. 2010

BP plasmid vector backbones are derived from pBPGUw. They contain the pUC19-derived bacterial origin of replication and ampicillin resistance gene, the PhiC31 attB site, the mini-white marker for identification of transformants in Drosophila, and the DSCP basal promoter. Abbreviations: U, DSCP basal promoter; w, mini-white marker; nls, nuclear localization signal; IVS, intervening sequence within the 5’ UTR; WPRE, a Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element within the 3’ UTR; 3’ UTR Term., the transcriptional terminator; and pBP, plasmid BP backbone.

Janelia Reporter Construct (JFRC) backbones are derived from pBDP. In addition, all vectors also contain a basal promoter derived from hsp70 and an SV40 transcriptional terminator.

a = Drosophila codon-optimized transgene

Plasmids from Nern et al. PNAS 2011: Multiple new site-specific recombinases for use in manipulating animal genomes.

Name	Addgene Plasmid ID
pJFRC150-20XUAS-IVS-Flp1::PEST	32132
pJFRC151-3XUAS-IVS-Flp2::PEST	32133
pJFRC153-20XUAS-IVS-B2::PEST	32134
pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP	32135
pJFRC157-20XUAS-IVS-B3::PEST	32136
pJFRC159-1XUAS-DSCP-B3::PEST	32137
pJFRC158-3XUAS-IVS-B3::PEST	32138
pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP	32139
pJFRC161-20XUAS-IVS-KD::PEST	32140
pJFRC164-21XUAS-KDRT>-dSTOP-KDRT>-myr::RFP	32141
pJFRC165-20XUAS-IVS-R::PEST	32142
pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP	32143
pJFRC170-3XUAS-IVS-Cre::PEST	32144
pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP	32145
pJFRC173-20XUAS-IVS-Dre::PEST	32146
pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP	32147
pBPhsFlp1	32148
pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP	32149