Drosophilia Genetic Tools
About the Innovation
Rubin lab Plasmid Constructs are available from Addgene, which has distributed over 430 plasmids listed below.
Plasmids from Pfeiffer et al. 2008 and Pfeiffer et al. 2010.
Name | Addgene Plasmid ID | 5' UTR IVS | Transgene | 3' UTR WPRE | 3' UTR Term. | Ref |
---|---|---|---|---|---|---|
pBDP |
|
none |
|
|
||
pBPGUw |
|
GAL4a |
|
hsp70 |
||
pBPGw |
|
GAL4a |
|
hsp70 |
||
pBPGAL4.1Uw |
|
GAL4a |
|
hsp70 |
||
pBPGAL4.2Uw-2 |
|
GAL4a |
|
SV40 |
||
pBPGAL4.2::VP16Uw |
|
GAL4::VP16a |
|
hsp70 |
||
pBPGAL4.2::p65Uw |
|
GAL4::p65a |
|
hsp70 |
||
pBPnlsLexA::GADflUw |
|
nlsLexA::GADfla |
|
hsp70 |
||
pBPLexA::p65Uw |
|
LexA::p65a |
|
hsp70 |
||
pBPnlsLexA::p65Uw |
|
nlsLexA::p65a |
|
hsp70 |
||
pBPZpGAL4DBDUw |
|
Zip-GAL4DBDa |
|
hsp70 |
||
pBPp65ADZpUw |
|
p65AD-Zipa |
|
hsp70 |
||
pBPGAL80Uw-4 |
+ |
GAL80a |
+ |
hsp70 |
||
pBPGAL80Uw-6 |
+ |
GAL80a |
+ |
SV40 |
||
pJFRC-MUH |
|
|
|
|
||
pJFRC12-10XUAS-IVS-myr::GFP |
+ |
myr::GFP |
|
SV40 |
||
pJFRC14-10XUAS-IVS-GFP-WPRE |
+ |
GFP |
+ |
SV40 |
||
pJFRC18-8XLexAop2-mCD8::GFP |
|
mCD8::GFP |
|
SV40 |
||
pJFRC19-13XLexAop2-IVS-myr::GFP |
+ |
myr::GFP |
|
SV40 |
||
pJFRC2-10XUAS-IVS-mCD8::GFP |
+ |
mCD8::GFP |
|
SV40 |
||
pJFRC2-INS |
|
mCD8::GFP |
|
SV40 |
||
pJFRC3-1XUAS-IVS-mCD8::GFP |
|
mCD8::GFP |
|
SV40 |
||
pJFRC4-3XUAS-IVS-mCD8::GFP |
|
mCD8::GFP |
|
SV40 |
||
pJFRC5-5XUAS-IVS-mCD8::GFP |
|
mCD8::GFP |
|
SV40 |
||
pJFRC6-15XUAS-IVS-mCD8::GFP |
|
mCD8::GFP |
|
SV40 |
||
pJFRC7-20XUAS-IVS-mCD8::GFP |
|
mCD8::GFP |
|
SV40 |
||
pJFRC8-40XUAS-IVS-mCD8::GFP |
|
mCD8::GFP |
|
SV40 |
BP plasmid vector backbones are derived from pBPGUw. They contain the pUC19-derived bacterial origin of replication and ampicillin resistance gene, the PhiC31 attB site, the mini-white marker for identification of transformants in Drosophila, and the DSCP basal promoter. Abbreviations: U, DSCP basal promoter; w, mini-white marker; nls, nuclear localization signal; IVS, intervening sequence within the 5’ UTR; WPRE, a Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element within the 3’ UTR; 3’ UTR Term., the transcriptional terminator; and pBP, plasmid BP backbone.
Janelia Reporter Construct (JFRC) backbones are derived from pBDP. In addition, all vectors also contain a basal promoter derived from hsp70 and an SV40 transcriptional terminator.
a = Drosophila codon-optimized transgene
Plasmids from Nern et al. PNAS 2011: Multiple new site-specific recombinases for use in manipulating animal genomes.
Name | Addgene Plasmid ID |
---|---|
pJFRC150-20XUAS-IVS-Flp1::PEST |
|
pJFRC151-3XUAS-IVS-Flp2::PEST | |
pJFRC153-20XUAS-IVS-B2::PEST |
|
pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP |
|
pJFRC157-20XUAS-IVS-B3::PEST |
|
pJFRC159-1XUAS-DSCP-B3::PEST |
|
pJFRC158-3XUAS-IVS-B3::PEST |
|
pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP |
|
pJFRC161-20XUAS-IVS-KD::PEST |
|
pJFRC164-21XUAS-KDRT>-dSTOP-KDRT>-myr::RFP |
|
pJFRC165-20XUAS-IVS-R::PEST |
|
pJFRC168-21XUAS-RSRT>-dSTOP-RSRT>-myr::RFP |
|
pJFRC170-3XUAS-IVS-Cre::PEST |
|
pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP |
|
pJFRC173-20XUAS-IVS-Dre::PEST |
|
pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP |
|
pBPhsFlp1 |
|
pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP |
Plasmids from Pfeiffer et al. PNAS 2012: Using translational enhancers to increase transgene expression in Drosophila
Name | Addgene Plasmid ID |
---|---|
pJFRC28-10XUAS-IVS-GFP-p10 |
|
pJFRC81-10XUAS-IVS-Syn21-GFP-p10 |