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19 ResultsShowing 1-10 of 19 results
We have developed a widely used Cre-dependent viral vector utilizing a genetic element called a FLEX switch. Plasmids are available from Addgene.com...
CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that enables imaging of the integrated calcium activity of large populations of cells...
The second generation of CaMPARI molecules have several advantages over their predecessors. By mutating the amino acids in the photophore domain, researchers were able to improve green brightness...
The use of fluorescence technologies for biochemi-cal and biological research has exploded in recent years. Chemical fluorophores can enable super-resolution microscopy, high-throughput screening...
CASFISH: DNA labeling with CRISPR/Cas9
Genomic DNA in cells is highly folded and organized in three dimensions. Detection and visualization of genomic DNA is widely accomplished through the use of various ...
Chimeric Ligand-Gated Ion Channels
In the nervous system, ligand-gated ion channels (LGICs) transduce chemical signals into electrical activity that communicates information. Implicated in many neurological disorders, LGICs are...
Drosophilia Genetic Tools
Rubin lab Plasmid Constructs are available from Addgene, which has distributed over 430 of the plasmids listed below. Plasmids from Pfeiffer et al. 2008 and Pfeiffer...
Current Iterations: GCaMP3, GCaMP5, GCaMP6, jGCaMP7 Fluorescent calcium indicators are an important tool used to detect and measure calcium, an essential regulator of cellular signaling....
Inventors at Janelia have developed a strategy for the creation of genetically encoded, intensity-based fluorescent sensors and an associated unique set of analytical tools that can measure...
The major neurotransmitter in the brain, glutamate is an important signaling molecule in biological organisms. The ability to visualize and quantitate glutamate transients is broadly useful to...