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3 Janelia Publications

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    10/01/10 | Semi-automated reconstruction of neural circuits using electron microscopy.
    Chklovskii DB, Vitaladevuni S, Scheffer LK
    Current Opinion in Neurobiology. 2010 Oct;20:667-75. doi: 10.1371/journal.pcbi.1001066

    Reconstructing neuronal circuits at the level of synapses is a central problem in neuroscience, and the focus of the nascent field of connectomics. Previously used to reconstruct the C. elegans wiring diagram, serial-section transmission electron microscopy (ssTEM) is a proven technique for the task. However, to reconstruct more complex circuits, ssTEM will require the automation of image processing. We review progress in the processing of electron microscopy images and, in particular, a semi-automated reconstruction pipeline deployed at Janelia. Drosophila circuits underlying identified behaviors are being reconstructed in the pipeline with the goal of generating a complete Drosophila connectome.

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    01/01/10 | Increasing depth resolution of electron microscopy of neural circuits using sparse tomographic reconstruction.
    Veeraraghavan A, Genkin AV, Vitaladevuni S, Scheffer L, Xu C, Hess H, Fetter R, Cantoni M, Knott G, Chklovskii DB
    Computer Vision and Pattern Recognition (CVPR). 2010:1767-74. doi: 10.1109/CVPR.2010.5539846
    01/01/10 | Serial-section EM derived synaptic circuits in the fly’s visual system: the medulla opens up.
    Meinertzhagen IA, Takemura S, Vitaladevuni S, Lu Z, Scheffer L, Chklovskii D
    Journal of Neurogenetics. 2010;24:9