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1 Janelia Publications

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    Hess Lab
    01/26/14 | Correlative super-resolution fluorescence and metal-replica transmission electron microscopy.
    Sochacki KA, Shtengel G, Van Engelenburg SB, Hess HF, Taraska JW
    Nature Methods. 2014 Jan 26;11(3):305-8. doi: 10.1038/nmeth.2816

    We combine super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas to locate proteins on the landscape of the cellular plasma membrane at the nanoscale. We validate robust correlation on the scale of 20 nm by imaging endogenous clathrin (in two and three dimensions) and apply the method to find the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane.

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