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3 Janelia Publications

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    Ji Lab
    09/17/14 | The practical and fundamental limits of optical imaging in mammalian brains.
    Ji N
    Neuron. 2014 Sep 17;83(6):1242-1245. doi: 10.1016/j.neuron.2014.08.009

    Advances in chemistry and physics have profound effects on neuroimaging. Current and future progress in these disciplines will continue to aid in efforts to visualize neural circuitry, particularly in deeper layers of the brain.

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    Ji LabGENIE
    08/17/14 | Multiplexed aberration measurement for deep tissue imaging in vivo.
    Wang C, Liu R, Milkie DE, Sun W, Tan Z, Kerlin A, Chen T, Kim DS, Ji N
    Nature Methods. 2014 Aug 17:. doi: 10.1038/nmeth.3068

    We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

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    Ji Lab
    01/27/14 | Direct phase measurement in zonal wavefront reconstruction using multidither coherent optical adaptive technique.
    Liu R, Milkie DE, Kerlin A, Maclennan B, Ji N
    Optics Express. 2014 Jan 27;22(2):1619-28. doi: 10.1364/OE.22.001619

    In traditional zonal wavefront sensing for adaptive optics, after local wavefront gradients are obtained, the entire wavefront can be calculated by assuming that the wavefront is a continuous surface. Such an approach will lead to sub-optimal performance in reconstructing wavefronts which are either discontinuous or undersampled by the zonal wavefront sensor. Here, we report a new method to reconstruct the wavefront by directly measuring local wavefront phases in parallel using multidither coherent optical adaptive technique. This method determines the relative phases of each pupil segment independently, and thus produces an accurate wavefront for even discontinuous wavefronts. We implemented this method in an adaptive optical two-photon fluorescence microscopy and demonstrated its superior performance in correcting large or discontinuous aberrations.

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