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Wu LabTranscription Imaging
01/01/13 |
Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
Nature Methods. 2013;10(1):60-3. doi: 10.1038/nmeth.2277
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.