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1 Janelia Publications

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    02/03/20 | Cellpose: a generalist algorithm for cellular segmentation
    Stringer C, Michaelos M, Pachitariu M
    bioRxiv. 2020 Feb 03:. doi: 10.1101/2020.02.02.931238

    Many biological applications require the segmentation of cell bodies, membranes and nuclei from microscopy images. Deep learning has enabled great progress on this problem, but current methods are specialized for images that have large training datasets. Here we introduce a generalist, deep learning-based segmentation algorithm called Cellpose, which can very precisely segment a wide range of image types out-of-the-box and does not require model retraining or parameter adjustments. We trained Cellpose on a new dataset of highly-varied images of cells, containing over 70,000 segmented objects. To support community contributions to the training data, we developed software for manual labelling and for curation of the automated results, with optional direct upload to our data repository. Periodically retraining the model on the community-contributed data will ensure that Cellpose improves constantly.

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