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2 Janelia Publications

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    06/01/19 | Practical considerations in particle and object tracking and analysis.
    Aaron J, Wait E, DeSantis M, Chew T
    Current Protocols in Cell Biology. 2019 Jun 01;83(1):e88. doi: 10.1002/cpcb.88

    The rapid advancement of live-cell imaging technologies has enabled biologists to generate high-dimensional data to follow biological movement at the microscopic level. Yet, the "perceived" ease of use of modern microscopes has led to challenges whereby sub-optimal data are commonly generated that cannot support quantitative tracking and analysis as a result of various ill-advised decisions made during image acquisition. Even optimally acquired images often require further optimization through digital processing before they can be analyzed. In writing this article, we presume our target audience to be biologists with a foundational understanding of digital image acquisition and processing, who are seeking to understand the essential steps for particle/object tracking experiments. It is with this targeted readership in mind that we review the basic principles of image-processing techniques as well as analysis strategies commonly used for tracking experiments. We conclude this technical survey with a discussion of how movement behavior can be mathematically modeled and described. © 2019 by John Wiley & Sons, Inc.

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    07/01/17 | Opening a path to commercialization.
    Optics and Photonics News. 2017 Jul 01;28(7):42-9. doi: 10.1364/OPN.28.7.000042

    To smooth the academic-to-industry transition, one institution is experimenting with offering biomedical researchers pre-commercial open access to new optical imaging systems still under development. The approach, the authors of this case study suggest, can be a win on both sides.

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