| Release 3.0 (2017-09-01) |
- Images of brains and VNCs of 2,800 VT GAL4 lines have been added
in this release. The central brains of these lines had been imaged
previously in Vienna and these images can be viewed at
Virtual Fly Brain.
Here we add complete brains (with optic lobes) and VNCs imaged in the same way as the other
GAL4 lines shown on the site.
|
| Release 2.0 (2015-05-01) |
- A number of new GAL4 line images have been added, and
some corrections to the original data set have been made. These are listed
here.
- Image data for 1,479 LexA lines have been added (listed
here). These lines were generated
using the same enhancers and are generally inserted into the
attP40 landing site. Searching by line
name or gene name will recover both the available GAL4 and LexA image
data.
|
The data presented on this site are the work of the
Janelia FlyLight
Project Team and the laboratories of
Gerald M. Rubin,
James W. Truman,
Barry J. Dickson,
Richard S. Mann, and
Christopher Q. Doe.
For questions about sample creation/processing/imaging or about this website, please email
[email protected].
You may use images from this website under a
CC BY 4.0 license.
Please acknowledge the FlyLight Project Team at Janelia Research Campus for the use of the images.
Please also cite the appropriate papers listed below (often
Jennet et al. (2012) for Rubin GAL4 lines and
Tirian et al. (2017) for VT GAL4 lines).
The GMR GAL4 lines have been deposited in the
Bloomington Drosophila Stock Center
for distribution.
Discrepancies are likely to occur at some low frequency in a collection of
this size; if you obtain a line and find it does not match what is presented
on this site, please let us know by emailing
[email protected].
Genomic location coordinates for CRMs correspond to Flybase release 5.22,
which is available through the
Flybase Archive page.
Many of the VT lines are available from the
Vienna Drosophila Resource Center.
An Excel file containing details of PCR primers, predicted CRMs, neighboring genes, etc.
can also be downloaded from this site.
Many GAL4 and LexA lines are also available from the
Korea Drosophila Resource Center.
In publications, please attribute the data presented on this site to one of
the following papers, as follows:
For the overall strategy and methods used to produce the GMR GAL4 lines:
Pfeiffer, B. D., Jenett, A., Hammonds, A. S., Ngo, T. B., Misra, S., Murphy, C., Scully, A., Carlson, J. W., Wan, K. H., Laverty, T. R., Mungall, C., Svirskas, R., Kadonaga, J. T., Doe, C. Q., Eisen, M. B., Celniker, S. E., & Rubin, G. M. Tools for neuroanatomy and neurogenetics in Drosophila. Proceedings of the National Academy of Sciences. 2008; 105: 9715-9720.
DOI:
10.1073/pnas.0803697105
For the construction of the complete set of GAL4 lines and data on expression
in the adult nervous system:
Jenett, A., Rubin, G.M., Ngo, T.-T. B., Shepherd, D., Murphy, C., Dionne, H.,
Pfeiffer, B.D., Cavallaro, A., Hall, D., Jeter, J., Iyer, N.,
Fetter, D., Hausenfluck, J.H., Peng, H., Trautman, E., Svirskas, R., Myers, G.W.,
Iwinski, Z.R., Aso, Y., DePasquale, G.M., Enos, A., Hulamm, P., Lam, S.C.B.,
Li, H-H., Laverty, T., Long, F., Qu, L., Murphy, S.D., Rokicki, K., Safford, T.,
Shaw, K., Simpson, J.H., Sowell, A., Tae, S., Yu, Y., Zugates, C.T. A
GAL4-Driver Line Resource for Drosophila Neurobiology. Cell Reports (2012)
Doi: 10.1016/j.celrep.2012.09.011
http://www.cell.com/cell-reports/fulltext/S2211-1247(12)00292-6
For the construction of the split-GAL4
hemidriver collection based on Jenett, et al., 2012:
Dionne, H., Hibbard, K. L., Cavallaro, A., Kao, J. & Rubin, G. M. Genetic
Reagents for Making Split-GAL4 Lines in Drosophila. Genetics. 2018; 209:
31-35. DOI:
10.1534/genetics.118.300682
For data on expression in the larval nervous
system:
Li, H., Kroll, J. R., Lennox, S. M., Ogundeyi, O., Jeter, J., Depasquale, G., & Truman, J. W. A GAL4 Driver Resource for Developmental and Behavioral Studies on the Larval CNS of Drosophila. Cell Reports. 2014; 8: 897-908.
DOI:
10.1016/j.celrep.2014.06.065
For data on expression in the embryo:
Manning, L., Purice, M.D., Roberts, J., Pollard, J.L., Bennett, A.L., Kroll,
J.R., Dyukareva, A.V., Doan, P.N., Lupton, J.R., Strader, M.E., Tanner, S.,
Bauer, D., Wilbur, A., Tran, K.D., Laverty, T.R., Pearson, J.C., Crews, S.T.,
Rubin, G.M., and Doe, C.Q. (2012) Annotated embryonic CNS expression patterns
of 5000 GMR GAL4 lines: a resource for manipulating gene expression and
analyzing cis-regulatory motifs. Cell Reports (2012) Doi:
10.1016/j.celrep.2012.09.009
http://www.cell.com/cell-reports/fulltext/S2211-1247(12)00290-2
For data on expression in imaginal discs:
Jory, A., Estella, C., Giorgianni, M.W., Slattery, M., Laverty, T.R., Rubin,
G.M., and Mann, R.S. (2012) A survey of 6300 genomic fragments for
cis-regulatory activity in the imaginal discs of
Drosophila melanogaster.
Cell Reports (2012) Doi: 10.1016/j.celrep.2012.09.010
http://www.cell.com/cell-reports/fulltext/S2211-1247(12)00291-4
For construction of the VT GAL4 lines:
Tirian, L., & Dickson, B. J. The VT GAL4, LexA, and split-GAL4 driver line collections for targeted expression in the Drosophila nervous system. bioRxiv. 2017: 198648.
DOI:
10.1101/198648
