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GCaMP6 calcium indicators

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GCaMP6 calcium indicators
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GCaMP6 calcium indicators
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Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

CMV-GCaMP6s

improved SNR, slower kinetics

40753

Chen et al., 2013

CMV-GCaMP6m

improved SNR, intermediate kinetics

40754

Chen et al., 2013

CMV-GCaMP6f

improved SNR, faster kinetics

40755

Chen et al., 2013

 

Viruses available from University of Pennsylvania Viral Vector Core:

Name Penn Catalog Number Penn Map and Sequence Number Reference

AAV1.Syn.GCaMP6f.WPRE.SV40

AV-1-PV2822

Penn Vector P2822

Chen et al., 2013

AAV1.Syn.GCaMP6s.WPRE.SV40

AV-1-PV2824

Penn Vector P2824

Chen et al., 2013

AAV1.Syn.GCaMP6m.WPRE.SV40

AV-1-PV2823

Penn Vector P2823

Chen et al., 2013

AAV5.Syn.GCaMP6m.WPRE.SV40

AV-5-PV2823

Penn Vector P2823

Chen et al., 2013

AAV5.Syn.GCaMP6f.WPRE.SV40

AV-5-PV2822

Penn Vector P2822

Chen et al., 2013

AAV5.Syn.GCaMP6s.WPRE.SV40

AV-5-PV2824

Penn Vector P2824

Chen et al., 2013

AAV9.Syn.GCaMP6f.WPRE.SV40

AV-9-PV2822

Penn Vector P2822

Chen et al., 2013

AAV9.Syn.GCaMP6m.WPRE.SV40

AV-9-PV2823

Penn Vector P2823

Chen et al., 2013

AAV9.Syn.GCaMP6s.WPRE.SV40

AV-9-PV2824

Penn Vector P2824

Chen et al., 2013

AAV1.CAG.GCaMP6s.WPRE.SV40

AV-1-PV2833

Penn Vector P2833

Chen et al., 2013

AAV5.CAG.GCaMP6s.WPRE.SV40

AV-5-PV2833

Penn Vector P2833

Chen et al., 2013

AAV9.CAG.GCaMP6s.WPRE.SV40

AV-9-PV2833

Penn Vector P2833

Chen et al., 2013

AAV1.CAG.Flex.GCaMP6f.WPRE.SV40

AV-1-PV2816

Penn Vector 2816

Chen et al., 2013

AAV1.CAG.Flex.GCaMP6m.WPRE.SV40

AV-1-PV2817

Penn Vector 2817

Chen et al., 2013

AAV1.CAG.Flex.GCaMP6s.WPRE.SV40

AV-1-PV2818

Penn Vector 2818

Chen et al., 2013

AAV1.Syn.Flex.GCaMP6f.WPRE.SV40

AV-1-PV2819

Penn Vector 2819

Chen et al., 2013

AAV1.Syn.Flex.GCaMP6m.WPRE.SV40

AV-1-PV2820

Penn Vector 2820

Chen et al., 2013

AAV1.Syn.Flex.GCaMP6s.WPRE.SV40

AV-1-PV2821

Penn Vector 2821

Chen et al., 2013

AAV5.CAG.Flex.GCaMP6f.WPRE.SV40

AV-5-PV2816

Penn Vector 2816

Chen et al., 2013

AAV5.CAG.Flex.GCaMP6m.WPRE.SV40

AV-5-PV2817

Penn Vector 2817

Chen et al., 2013

AAV5.CAG.Flex.GCaMP6s.WPRE.SV40

AV-5-PV2818

Penn Vector 2818

Chen et al., 2013

AAV5.Syn.Flex.GCaMP6f.WPRE.SV40

AV-5-PV2819

Penn Vector 2819

Chen et al., 2013

AAV5.Syn.Flex.GCaMP6m.WPRE.SV40

AV-5-PV2820

Penn Vector 2820

Chen et al., 2013

AAV5.Syn.Flex.GCaMP6s.WPRE.SV40

AV-5-PV2821

Penn Vector 2821

Chen et al., 2013

AAV9.CAG.Flex.GCaMP6f.WPRE.SV40

AV-9-PV2816

Penn Vector 2816

Chen et al., 2013

AAV9.CAG.Flex.GCaMP6m.WPRE.SV40

AV-9-PV2817

Penn Vector 2817

Chen et al., 2013

AAV9.CAG.Flex.GCaMP6s.WPRE.SV40

AV-9-PV2818

Penn Vector 2818

Chen et al., 2013

AAV9.Syn.Flex.GCaMP6f.WPRE.SV40

AV-9-PV2819

Penn Vector 2819

Chen et al., 2013

AAV9.Syn.Flex.GCaMP6m.WPRE.SV40

AV-9-PV2820

Penn Vector 2820

Chen et al., 2013

AAV9.Syn.Flex.GCaMP6s.WPRE.SV40

AV-9-PV2821

Penn Vector 2821

Chen et al., 2013

 

 

Mice available from The Jackson Laboratory:

Name Description Jax Stock Number Reference

Thy1-GCaMP6s-WPRE (founder line GP4.3)

improved SNR, slower kinetics, variable neuronal expression pattern

024275

Dana et al., 2015

Thy1-GCaMP6s-WPRE (founder line GP4.6) improved SNR, slower kinetics, variable neuronal expression pattern 028278 Dana et al., 2015

Thy1-GCaMP6s-WPRE (founder line GP4.12)

improved SNR, slower kinetics, variable neuronal expression pattern

025776

Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.1) improved SNR, faster kinetics, variable neuronal expression pattern 028279 Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.3) improved SNR, faster kinetics, variable neuronal expression pattern 028280 Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.5)

improved SNR, faster kinetics, variable neuronal expression pattern

024276

Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.11)

improved SNR, faster kinetics, variable neuronal expression pattern

024339

Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.12) improved SNR, faster kinetics, variable neuronal expression pattern 028281 Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.17)

improved SNR, faster kinetics, variable neuronal expression pattern

025393

Dana et al., 2015

 

 

Flies available from Bloomington Stock Center:

Line name Insertion Site Bloomington Stock Number Reference Chr
P{20XUAS-IVS-GCaMP6s} attP40 42476 Chen et al., 2013 2
P{20XUAS-IVS-GCaMP6m} attP40 42748 Chen et al., 2013 2
P{20XUAS-IVS-GCaMP6f} attP40 42747 Chen et al., 2013 2
PBac{20XUAS-IVS-GCaMP6s} VK00005 42749 Chen et al., 2013 3
PBac{20XUAS-IVS-GCaMP6m} VK00005 42750 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6s-SV40} su(Hw)attP1 44273 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6m-SV40} su(Hw)attP1 44588 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6s-SV40} su(Hw)attP5 44589 Chen et al., 2013 2
P{13XLexAop2-IVS-GCaMP6s-p10} su(Hw)attP1 44274 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6m-p10} su(Hw)attP1 44275 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6s-p10} su(Hw)attP5 44590 Chen et al., 2013 2
P{13XLexAop2-IVS-GCaMP6f-p10} su(Hw)attP5 44277 Chen et al., 2013 2
P{13XLexAop2-IVS-GCaMP6m-p10} VK00005 44276 Chen et al., 2013 3

 

C. elegans distributed upon request (contact Program Scientist):

Name

mec-4::nls-RSET-GCaMP6s:SL2:nls-TagRFP::unc-54utr