Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.

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Nature Methods. 2013;10(1):60-3. doi: 10.1038/nmeth.2277
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Fast multicolor 3D imaging using aberration-corrected multifocus microscopy. Wu LabTranscription Imaging
Abrahamsson S, Chen J, Hajj B, Stallinga S, Katsov AY, Wisniewski J, Mizuguchi G, Soule P, Mueller F, Darzacq CD, Darzacq X, Wu C, Bargmann CI, Agard DA, Dahan M, Gustafsson MG
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Abstract

Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

PMID: 23223154 [PubMed - indexed for MEDLINE]

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Janelia Authors

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