New insights and system designs for temporally focused multiphoton optogenetics.

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Proceedings of SPIE. 2015 Mar 5;9329:932928. doi: 10.1117/12.2078678
New insights and system designs for temporally focused multiphoton optogenetics. GENIE
Mayblum T, Schejter A, Dana H, Shoham S
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Abstract

Temporal focusing (TF) multiphoton systems constitute a powerful solution for cellular resolution optogenetic stimulation and recording in three-dimensional, scattering tissue. Here, we address two fundamental aspects in the design of such systems: first, we examine the design of TF systems with specific optical sectioning by comparatively analyzing previously published results. Next, we develop a solution for obtaining TF in a flexible three-dimensional pattern of cellmatched focal spots. Our solution employs spatio-temporal focusing (SSTF) in a unique optical system design that can be integrated before essentially any multiphoton imaging or stimulation system.

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Janelia Authors

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