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Methods in Molecular Biology. 2021 May 28;2304:339-346. doi: 10.1007/978-1-0716-1402-0_18
FLIM Imaging for Metabolic Studies in Live Cells.
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Abstract
Fluorescent biochemical sensors allow probing metabolic states in a living cell with high spatiotemporal dynamics. This chapter describes a method for the in situ detection of changes in NAD level in living cells using fluorescence lifetime imaging (FLIM).
PMID: 34028726 [PubMed - indexed for MEDLINE]
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Janelia Authors
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