BAPTA-JF: Bright and Targetable Ca²⁺ Indicators

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BAPTA-JF: Bright and Targetable Ca²⁺ Indicators

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Bright and Targetable Red Ca²⁺ Indicators with JF-BAPTA Technology

Overview

BAPTA-JF indicators merge the superior properties of the Janelia Fluor® dyes, the Ca²⁺ sensitivity of BAPTA, and the genetic targeting precision of self-labeling tags like the HaloTag. This next-generation sensor platform delivers high-performance for both cellular and subcellular functional imaging.

Key Features

High ΔF/F₀ and Quantum Yield: Up to 15-fold ΔF/F₀ and quantum yields as high as 0.75.
Compatible with HaloTag: Enables genetically defined labeling and precise subcellular imaging.
Spectral Flexibility: JF₅₄₉ and JF₆₄₆ derivatives offer compatibility with GFP, optogenetics, and deep-tissue imaging setups.
Far-Red Imaging of the Primary Cilium: First small-molecule red calcium dye to successfully label and detect dynamic calcium fluxes in the primary cilium.

Spotlight Indicators

BAPTA-JF₅₄₉: Bright red-shifted indicator for general Ca²⁺ imaging; K_d = 310 nM; ΔF/F₀ = 15; φ_f = 0.75.
BAPTA-JF₅₄₉-HaloTag ligand: Targetable red-shifted indicator using the HaloTag system; K_d = 290 nM; ΔF/F₀ = 13.8; φ_f = 0.74.
BAPTA-JF₆₄₆-HaloTag ligand: Far-red, fluorogenic probe ideal for subcellular and deep tissue imaging; K_d = 140 nM; ΔF/F₀ = 5.5; φ_f = 0.55.