Bright, Photoactivatable (PA) Janelia Fluor® Dyes for Super-Resolution Imaging

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Bright, Photoactivatable (PA) Janelia Fluor® Dyes for Super-Resolution Imaging

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Photoactivatible Janelia Fluor® Dyes

Overview

Photoactivatable versions of Janelia Fluor® dyes combine the brightness and photostability of this small-molecule fluorophore platform with the spatial and temporal control of photoactivation, ideal for advanced imaging workflows.

Key Advantages

On-Demand Activation: Light-controlled activation with UV or violet (405 nm) light enables photoactivated localization microscopy (PALM), single-particle tracking PALM (sptPALM).
Exceptional Brightness: The PA-JF dyes retain the properties of the parent Janelia Fluor® compounds, including high quantum yields and photostability.
Live-Cell and Fixed-Cell Compatibility: Easily label intracellular targets using the HaloTag, SNAP-tag, or other labeling systems.
Multicolor Capability: Distinct spectral windows for PA-JF549 (~550 nm excitation) and PA-JF646 (~640 nm excitation) enable simultaneous two-color imaging.

Spotlight Dyes

PA-JF₅₄₉: Bright, fast-activating dye for single-molecule and high-precision localization microscopy.
PA-JF₆₄₆: Red-shifted dye for deep-tissue and two-color super-resolution imaging alongside green/orange channels.

Applications

Live-cell PALM and sptPALM
Single particle tracking of nuclear and cytoplasmic proteins
Super-resolution imaging of cellular structures

IP Coverage: US Patent 11,958,976, JP Application 2018-560894, EP Application EP17800333.1, CN 2017800283784

Reference: Nat. Methods 2016, 13, 985-988, https://doi.org/10.1038/nmeth.4034.

Opportunity: Available for non-exclusive commercial license

Janelia Tech ID: 2016-034

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Bright photoactivatable fluorophores for single-molecule imaging.

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Bright, Photoactivatable (PA) Janelia Fluor® Dyes for Super-Resolution Imaging