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Main Menu - Block
- Overview
- Anatomy and Histology
- Cryo-Electron Microscopy
- Electron Microscopy
- Flow Cytometry
- Fly Facility
- Gene Targeting and Transgenics
- Immortalized Cell Line Culture
- Integrative Imaging
- Janelia Experimental Technology
- Media Prep
- Molecular Genomics
- Primary & iPS Cell Culture
- Project Pipeline Support
- Project Technical Resources
- Quantitative Genomics
- Scientific Computing Software
- Scientific Computing Systems
- Viral Tools
- Vivarium
iGluSnFR3
Improved Glutamate Indicators for Synaptic Imaging
iGluSnFR3 is the current (2022) generation of Glutamate-Sensing Fluorescent Reporters developed at HHMI's Janelia Research Campus to image the most abundant neurotransmitter in the mammal brain with the greatest performance to date.
Fig 1. iGluSnFR mechanism of action
Performance
Building on the previous generation of the state-of-the-art SF-iGluSnFR scaffold (itself an improvement over earlier versions), Janelia Researchers, led by Abhi Aggarwal and Kaspar Podgorski, have further improved specificity for synaptic versus extrasynaptic glutamate. iGluSnFr3 variants are more sensitive, faster, and better localized for synaptic glutamate response than its predecessors. The report showing performance, experimental protocols and more is available on bioRxiv.
Fig 2. iGluSnFR3 variants ∆F/F0 performance
Fig 3. in vivo patch+ performance vs. previous generation
Availability
Two sensors with unique kinetics, affinity, and ∆F/F0 are available, v82 and v857, in three membrane-targeting backbones: PDGFR, GPI, and SGZ. These variants are available with Syn and CAG promoters, in plasmids and AAVs - listed below. These can be obtained directly from Addgene.
To request other AAVs or to make special requests for plasmids, you can contact Abhi Aggarwal or Kaspar Podgorski.
| Plasmid | Promoter | Variant |
| pRSET | T7 | pRSET.iGluSnFR3.v82 |
| pRSET | T7 | pRSET.iGluSnFR3.v857 |
| pAAV | hSyn | pAAV.hSyn(FLEX).iGluSnFR3.v82.GPI |
| pAAV | hSyn | pAAV.hSyn(FLEX).iGluSnFR3.v857.GPI |
| pAAV | hSyn | pAAV.hSyn(FLEX).iGluSnFR3.v857.PDGFR |
| pAAV | hSyn | pAAV.hSyn(FLEX).iGluSnFR3.v857.SGZ |
| pAAV | hSyn | pAAV.hSyn.iGluSnFR3.v857.SGZ |
| pAAV | CAG | pAAV.CAG.iGluSnFR3.v857.GPI |
| pAAV | CAG | pAAV.CAG.iGluSnFR3.v857.SGZ |
| pAAV | CAG | pAAV.CAG.iGluSnFR3.v857.PDGFR |
| pAAV | GFAP | pAAV.GFAP.iGluSnFR3.v857.SGZ |
| pAAV | GFAP | pAAV.GFAP.iGluSnFR3.v857.PDGFR |
Opportunity
Available for non-profit researchers at Addgene links above and to the right or by request to GENIE. Commercial licenses (for internal research or commercial sale) are also available - please contact innovation@janelia.hhmi.org.
Janelia Tech ID
2021-017
Intellectual Property coverage:
