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Photochromic Super-Resolution Microscopy Dyes
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Photochromic Janelia Fluor - dyes with a universal coumarin switch
Download our Janelia Fluor® Dye Catalog here.
Overview
A versatile platform enabling the construction of dyes for super-resolution imaging by coupling a coumarin auxiliary to rhodamine dyes. Dyes can be tuned to be either photochromic, with 405 nm activation, or spontaneously blinking at physiological conditions, yielding a palette of dyes in different colors.
Key Advantages
- Universal Switching Strategy: One chemical modification generates two classes of functional dyes for imaging.
- Predictable Behavior: Dye blinking or photoactivation outcomes based on parent dye KL–Z values.
- Live-Cell and Fixed-Cell Compatibility: Works under normal biological conditions without specialized buffers.
- Super-Resolution Ready: Optimized for SMLM, SOFI, and related single-molecule imaging modalities.
Spotlight Dyes
- GB646: Hybrid JF646–coumarin photochromic dye activated by 405 nm for super-resolution imaging.
- GB549: JF549–coumarin fluorophore that spontaneously blinks when conjugated to the HaloTag, eliminating the need for switching buffers.
- GB594: Alexa Fluor–coumarin dye that spontaneously blinks, allowing super-resolution imaging with antibody conjugates.
Applications
- Single-molecule localization microscopy (SMLM) in live and fixed cells
- Multiplexed imaging based on temporal and spectral signatures
Reference: bioRxiv 2024, https://doi.org/10.1101/2024.05.12.593749
IP Coverage: US Patent 11,067,566
Opportunity: Available for non-exclusive commercial licensing.
Janelia Tech ID: 2017-026
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GB594-Phalloidin. Courtesy of Lavis Lab.
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