Red-Shifted and Fluorinated Dyes "JF3" for Advanced Bioimaging

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Red-Shifted and Fluorinated Dyes "JF3" for Advanced Bioimaging

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JF3 - Red-shifted, Fluorinated Janelia Fluor Dyes

Overview

Fluorination and structural tuning of rhodamine scaffolds have yielded a powerful new class of bright, red-shifted fluorescent dyes for live-cell and super-resolution microscopy.

Key Advantages

Red/NIR Absorption and Emission: Fluorination shifts absorbance and emission by 15–25 nm, ideal for multiplexed imaging and deep-tissue applications.
Enhanced Brightness and Stability: Improved extinction coefficients (ε) and stable fluorescence performance for single-particle tracking (SPT) and FLIM.
Improved Bioavailability: Tuning the lactone–zwitterion equilibrium (K_L–Z) ensures excellent membrane permeability and fluorogenic behavior.
Flexible Functionalization: New synthetic strategies (e.g., masked acyl cyanide chemistry) simplify attachment to tags like HaloTag or SNAP-tag.

Spotlight Dyes

JF657: Fluorinated analog of ATTO 647N; excels in live-cell imaging and SPT with superior brightness and localization stability.
JF698: Near-infrared absorbing dye designed for minimal background and high sensitivity imaging.
JF632 / JFX637: Fluorinated carborhodamines for advanced fluorescence microscopy and sensing.
Additional dyes: JF563, JF610, JF669, JF593