A series of spontaneously blinking dyes for super-resolution microscopy

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bioRxiv. 2024 Feb 24;. doi: 10.1101/2024.02.23.581625

A series of spontaneously blinking dyes for super-resolution microscopy Lavis LabSinger LabTool Translation Team (T3)

Holland Katie, Plutkis Sarah, Daugird Timothy, Sau Abhishek, Grimm Jonathan, English Brian, Zheng Qinsi, Dave Sandeep, Rahman Fariha, Xie Liangqi, Dong Peng, Tkachuk Ariana, Brown Timothy, Singer Robert, Liu Zhe, Galbraith Catherine, Musser Siegfried, Legant Wesley, Lavis Luke

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Abstract

Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.

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bioRxiv preprint: http://dx.doi.org/10.1101/2024.02.23.581625

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