A Yeast Two-Hybrid Assay to Develop Optogenetic Tools for Controlling Protein-Protein Interactions.

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Methods Mol Biol. 2026 Aug 02;3032:69-83. doi: 10.1007/978-1-0716-5257-2_5
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A Yeast Two-Hybrid Assay to Develop Optogenetic Tools for Controlling Protein-Protein Interactions.
Woloschuk RM, Woolley GA
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Abstract

Yeast two hybrid (Y2H) assays serve as powerful platforms for the detection and engineering of protein-protein interactions. Here, we describe a strategy for using Y2H-based screening to assess and develop optogenetic tools based on light-controlled protein-protein interactions.

PMID: 42681176 [PubMed - indexed for MEDLINE]

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Janelia Authors

Ryan Woloschuk
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