Activity correlation imaging: visualizing function and structure of neuronal populations.

Our Research / Publications

janelia7_blocks-janelia7_biblio_header | block

Biophysical Journal. 2009 May 6;96(9):3801-9. doi: 10.1016/j.bpj.2008.12.3962

View Article on

Junek S, Chen T, Alevra M, Schild D

Note: Research in this publication was not performed at Janelia.

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

For the analysis of neuronal networks it is an important yet unresolved task to relate the neurons’ activities to their morphology. Here we introduce activity correlation imaging to simultaneously visualize the activity and morphology of populations of neurons. To this end we first stain the network’s neurons using a membrane-permeable [Ca(2+)] indicator (e.g., Fluo-4/AM) and record their activities. We then exploit the recorded temporal activity patterns as a means of intrinsic contrast to visualize individual neurons’ dendritic morphology. The result is a high-contrast, multicolor visualization of the neuronal network. Taking the Xenopus olfactory bulb as an example we show the activities of the mitral/tufted cells of the olfactory bulb as well as their projections into the olfactory glomeruli. This method, yielding both functional and structural information of neuronal populations, will open up unprecedented possibilities for the investigation of neuronal networks.

PMID: 19413986 [PubMed - indexed for MEDLINE]

node:body | entity_field

Main Menu (Mobile)- Block

Main Menu - Block

Activity correlation imaging: visualizing function and structure of neuronal populations.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 19413986 [PubMed - indexed for MEDLINE]

Janelia Authors