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Scientific Reports. 2014 Jan 9;4:3615. doi: 10.1038/srep03615
Background free imaging of single mRNAs in live cells using split fluorescent proteins.
Note: Research in this publication was not performed at Janelia.
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Abstract
We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.
PMID: 24402470 [PubMed - indexed for MEDLINE]
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Janelia Authors
Robert Singer
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