A bright and photostable photoconvertible fluorescent protein.

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Nature Methods. 2009 Feb;6(2):131-3. doi: 10.1038/nmeth.1296
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A bright and photostable photoconvertible fluorescent protein. Looger Lab
McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
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Abstract

Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the approximately 10-nm localization precision of its parent.

PMID: 19169260 [PubMed - indexed for MEDLINE]

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Janelia Authors

Loren Looger
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