Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.

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Cell. 2020 Jul;180(2):373-386.e15. doi: 10.1016/j.cell.2019.12.029

Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators. Li Lab

Li J, Han S, Li H, Udeshi ND, Svinkina T, Mani DR, Xu C, Guajardo R, Xie Q, Li T, Luginbuhl DJ, Wu B, McLaughlin CN, Xie A, Kaewsapsak P, Quake SR, Carr SA, Ting AY, Luo L

Note: Research in this publication was not performed at Janelia.

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Abstract

Molecular interactions at the cellular interface mediate organized assembly of single cells into tissues and, thus, govern the development and physiology of multicellular organisms. Here, we developed a cell-type-specific, spatiotemporally resolved approach to profile cell-surface proteomes in intact tissues. Quantitative profiling of cell-surface proteomes of Drosophila olfactory projection neurons (PNs) in pupae and adults revealed global downregulation of wiring molecules and upregulation of synaptic molecules in the transition from developing to mature PNs. A proteome-instructed in vivo screen identified 20 cell-surface molecules regulating neural circuit assembly, many of which belong to evolutionarily conserved protein families not previously linked to neural development. Genetic analysis further revealed that the lipoprotein receptor LRP1 cell-autonomously controls PN dendrite targeting, contributing to the formation of a precise olfactory map. These findings highlight the power of temporally resolved in situ cell-surface proteomic profiling in discovering regulators of brain wiring.

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Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 31955847 [PubMed - indexed for MEDLINE]

Janelia Authors