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Biochemistry. 2017 Jul 13;56(39):5165-70. doi: 10.1021/acs.biochem.7b00529
Chemistry is dead. Long live chemistry! Lavis Lab
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Abstract
Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the “photon budget” requires better and brighter fluorophores. Here, we review the recent advances in biochemistry, protein engineering, and organic synthesis that have allowed a triumphant return of chemical dyes to modern biological imaging.
PMID: 28704030 [PubMed - indexed for MEDLINE]
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Janelia Authors
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