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ChIP for hox proteins from Drosophila imaginal discs.

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Methods in Molecular Biology. 2014 August 15;1196:241-53. doi: 10.1007/978-1-4939-1242-1_15
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ChIP for hox proteins from Drosophila imaginal discs.
Agrawal P, Shashidhara LS
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Abstract

Chromatin immunoprecipitation (ChIP) is a technique that reveals in vivo location of a protein bound to DNA. ChIP coupled with DNA microarrays (ChIP-chip) or next-generation sequencing (ChIP-seq) allows for identification of binding sites of transcription factors on a global scale. Here we describe a protocol for ChIP to identify binding of the Ultrabithorax (Ubx) Hox transcription factors from imaginal discs of Drosophila larvae. The protocol can be extended to other model organisms and transcription factors.

PMID: 25151168 [PubMed - indexed for MEDLINE]

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