Frealign: an exploratory tool for single-particle cryo-EM.

janelia7_blocks-janelia7_biblio_header | block
Methods in Enzymology. 2016 Jun 07;. doi: 10.1016/bs.mie.2016.04.013
View Article on
Frealign: an exploratory tool for single-particle cryo-EM. Grigorieff Lab
Grigorieff N
janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Frealign is a software tool designed to process electron microscope images of single molecules and complexes to obtain reconstructions at the highest possible resolution. It provides a number of refinement parameters and options that allow users to tune their refinement to achieve specific goals, such as masking to classify selected regions within a particle, control over the refinement of specific alignment parameters to accommodate various data collection schemes, refinement of pseudosymmetric particles, and generation of initial maps. This chapter provides a general overview of Frealign functions and a more detailed guide to using Frealign in typical scenarios.

PMID: 27572728 [PubMed - indexed for MEDLINE]

node:body | entity_field
janelia7_blocks-janelia7_biblio_authors | block

Janelia Authors

janelia7_blocks-janelia7_biblio_tools | block