A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells.

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Bioorganic & Medicinal Chemistry Letters. 2008 Nov 15;18(22):5864-6. doi: 10.1016/j.bmcl.2008.06.015

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A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells. Lavis Lab

Yatzeck MM, Lavis LD, Chao T, Chandran SS, Raines RT

Note: Research in this publication was not performed at Janelia.

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Abstract

A derivative of rhodamine 110 has been designed and assessed as a probe for cytochrome P450 activity. This probe is the first to utilize a ’trimethyl lock’ that is triggered by cleavage of an ether bond. In vitro, fluorescence was manifested by the CYP1A1 isozyme with k(cat)/K(M)=8.8x10(3)M(-1)s(-1) and K(M)=0.09microM. In cellulo, the probe revealed the induction of cytochrome P450 activity by the carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin, and its repression by the chemoprotectant resveratrol.

PMID: 18595692 [PubMed - indexed for MEDLINE]

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A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 18595692 [PubMed - indexed for MEDLINE]

Janelia Authors