Imaging neuronal voltage beyond the scattering limit

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Nat Methods. 2025 May 19;. doi: 10.1038/s41592-025-02692-5

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Imaging neuronal voltage beyond the scattering limit Lavis Lab

Chen Tsai-Wen, Bin Huang Xian-, Plutkis Sarah, Holland Katie, Lavis Luke, Lin Bei-Jung

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Abstract

Voltage imaging is a promising technique for high-speed recording of neuronal population activity. However, tissue scattering severely limits its application in dense neuronal populations. Here, we adopted the principle of localization microscopy, a technique that enables super-resolution imaging of single-molecules, to resolve dense neuronal activities in vivo. Leveraging the sparse activation of neurons during action potentials (APs), we precisely localize the fluorescence change associated with each AP, creating a super-resolution image of neuronal activities. This approach, termed Activity Localization Imaging (ALI), identifies overlapping neurons and separates their activities with over 10-fold greater precision than what tissue scattering permits. Using ALI, we simultaneously recorded over a hundred densely-labeled CA1 neurons, creating a map of hippocampal theta oscillation at single-cell and single-cycle resolution.

Preprint: https://doi.org/10.1101/2023.12.03.56840

PMID: 40389606 [PubMed - indexed for MEDLINE]

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bioRxiv Preprint https://doi.org/10.1101/2023.12.03.568403

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Imaging neuronal voltage beyond the scattering limit

Abstract

PMID: 40389606 [PubMed - indexed for MEDLINE]

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