Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics

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Nat Methods. 2026 Jun 01;. doi: 10.1038/s41592-026-03076-z

Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics Harris Lab

Lakunina A, Socha KZ, Ladd A, Bowen AJ, Chen S, Colonell J, Doshi A, Karsh B, Krumin M, Kulik P, Li A, Neutens P, O’Callaghan J, Olsen M, Putzeys J, Tilmans HA, Ye Z, Welkenhuysen M, Häusser M, Koch C, Ting JT, Consortium Neuropixels, Dutta B, Harris TD, Steinmetz NA, Svoboda K, Siegle JH, Carandini M

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Abstract

High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations and is especially powerful when combined with optogenetics for manipulation of specific cell types with high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic and photonic circuits. These devices pack 960 electrical recording sites and two sets of 14 light emitters onto a 70-μm-wide, 1-cm-long shank, allowing spatially addressable optogenetic stimulation with blue and red light. In mouse cortex, Neuropixels Opto probes delivered high-quality recordings together with spatially addressable optogenetics, differentially activating or silencing neurons at distinct cortical depths. In the mouse striatum and other deep structures, Neuropixels Opto probes delivered efficient optotagging, facilitating the identification of two cell types in parallel. Neuropixels Opto probes represent a promising tool for recording, identifying and manipulating neuronal populations.