Quantitative mRNA imaging throughout the entire Drosophila brain.

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Nature Methods. 2017 Jun 05;14(7):703-6. doi: 10.1038/nmeth.4309
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Quantitative mRNA imaging throughout the entire Drosophila brain. Harris LabSinger LabTranscription ImagingFly Functional Connectome
Long X, Colonell J, Wong AM, Singer RH, Lionnet T
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Abstract

We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).

PMID: 28581495 [PubMed - indexed for MEDLINE]

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Janelia Authors

Jennifer Colonell
Robert Singer
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