Main Menu (Mobile)- Block
Main Menu - Block
janelia7_blocks-janelia7_biblio_header | block
Nature Methods. 2014 Dec;11(12):1237-41. doi: 10.1038/nmeth.3146
Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology. Harris Lab
janelia7_blocks-janelia7_biblio_abstract | block
Abstract
Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.
PMID: 25326662 [PubMed - indexed for MEDLINE]
node:body | entity_field
janelia7_blocks-janelia7_biblio_authors | block
Janelia Authors
janelia7_blocks-janelia7_biblio_tools | block