Reconstruction of complementary images in second harmonic generation microscopy.

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Optics Express. 2006 May 29;14(11):4727-35. doi: 10.1364/AO.50.001792

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Gao L, Jin L, Xue P, Xu J, Wang Y, Ma H, Chen D

Note: Research in this publication was not performed at Janelia.

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Abstract

Second harmonic generation microscopy(SHGM) has become widely used to image biological samples. Due to the complexity of biological samples, more and more effort has been put on polarization imaging in SHGM technology to uncover their structures. In this work, we put forward a novel stitching method based on careful mathematical calculation, and accomplish it by rotating laser polarization. We first show its validity in imaging a perfectly synthesized bio-origin polymer poly (3-hyroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). Then, we test its power by getting a true image of fibrillar collagen structure of rat-tail tendon.

PMID: 19516629 [PubMed - indexed for MEDLINE]

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Reconstruction of complementary images in second harmonic generation microscopy.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 19516629 [PubMed - indexed for MEDLINE]