Resolution in super-resolution microscopy - definition, trade-offs and perspectives.

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Nat Rev Mol Cell Biol. 2024 Jul 01;. doi: 10.1038/s41580-024-00755-7

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Resolution in super-resolution microscopy - definition, trade-offs and perspectives. Shroff Lab

Prakash K, Baddeley D, Eggeling C, Fiolka R, Heintzmann R, Manley S, Radenovic A, Smith C, Shroff H, Schermelleh L

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Abstract

Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.

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Resolution in super-resolution microscopy - definition, trade-offs and perspectives.

Abstract

PMID: 38951703 [PubMed - indexed for MEDLINE]

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