Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.

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Nature Communications. 2017 Nov 10;8(1):1412. doi: 10.1038/s41467-017-01752-5
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Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis. Lavis Lab
Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D
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Abstract

The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Orange that match the properties of SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization of both exocytosis and endocytosis, constituting new bright sensors for these key steps of synaptic transmission.

PMID: 29123102 [PubMed - indexed for MEDLINE]

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Janelia Authors

Jonathan Grimm
Luke Lavis
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