Single-synapse ablation and long-term imaging in live C. elegans.

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J Neurosci Methods. 2008 Jul;173(1):20-6. doi: 10.1016/j.jneumeth.2008.05.007

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Single-synapse ablation and long-term imaging in live C. elegans. Sgro Lab

Allen PB, Sgro AE, Chao DL, Doepker BE, Edgar J, Shen K, Chiu DT

Note: Research in this publication was not performed at Janelia.

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Abstract

Synapses are individually operated, computational units for neural communication. To manipulate physically individual synapses in a living organism, we have developed a laser ablation technique for removing single synapses in live neurons in C. elegans that operates without apparent damage to the axon. As a complementary technique, we applied microfluidic immobilization of C. elegans to facilitate long-term fluorescence imaging and observation of neuronal development. With this technique, we directly demonstrated the existence of competition between developing synapses in the HSNL motor neuron.

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Single-synapse ablation and long-term imaging in live C. elegans.

Note: Research in this publication was not performed at Janelia.

Abstract

PMID: 18579213 [PubMed - indexed for MEDLINE]

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