Super-resolution 3D microscopy of live whole cells using structured illumination.

janelia7_blocks-janelia7_biblio_header | block
Nature Methods. 2011 Dec;8:1044-6. doi: 10.1038/nmeth.1734
View Article on
Super-resolution 3D microscopy of live whole cells using structured illumination.
Shao L, Kner P, Rego EH, Gustafsson MG
janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Three-dimensional (3D) structured-illumination microscopy (SIM) can double the lateral and axial resolution of a wide-field fluorescence microscope but has been too slow for live imaging. Here we apply 3D SIM to living samples and record whole cells at up to 5 s per volume for >50 time points with 120-nm lateral and 360-nm axial resolution. We demonstrate the technique by imaging microtubules in S2 cells and mitochondria in HeLa cells.

PMID: 22002026 [PubMed - indexed for MEDLINE]

node:body | entity_field
janelia7_blocks-janelia7_biblio_authors | block

Janelia Authors

janelia7_blocks-janelia7_biblio_tools | block