Two-parameter single-molecule analysis for measurement of chromatin mobility.

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STAR Protocols. 2020 Dec 13;1(3):100223. doi: 10.1016/j.xpro.2020.100223
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Two-parameter single-molecule analysis for measurement of chromatin mobility. Liu (Zhe) Lab
Lerner J, Gomez-Garcia PA, McCarthy RL, Liu Z, Lakadamyali M, Zaret KS
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Abstract

This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).

PMID: 33377115 [PubMed - indexed for MEDLINE]

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Janelia Authors

Zhe J. Liu
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