RNase MRP correctly cleaves a novel R loop at the mitochondrial DNA leading-strand origin of replication.

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Genes & Development. 1997 Mar;11(5):582-92
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RNase MRP correctly cleaves a novel R loop at the mitochondrial DNA leading-strand origin of replication.
Clayton DA, Lee DY
Note: Research in this publication was not performed at Janelia.
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Abstract

The precursor primer RNA for mammalian mitochondrial DNA leading-strand replication remains as a persistent R loop formed during transcription through the mitochondrial DNA control region. We have examined model R loops, which exist in a novel and physiologically accurate preprimer conformation, as potential substrates for mammalian RNase mitochondrial RNA processing (MRP). Mouse RNase MRP accurately cleaves an R loop containing the mouse mitochondrial DNA origin. The multiple cleavage sites on the R-loop substrate match the priming sites observed in vivo, suggesting that RNase MRP alone is capable of generating virtually all of the leading-strand replication primers.

PMID: 9119223 [PubMed - indexed for MEDLINE]

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