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What We Do
Our Gene Targeting & Transgenic Facility makes sophisticated gene targeting a turnkey service at a low cost. We develop and experiment with new technologies to support research needs. We design and produce gene targeting, CRISPR/Cas9 and BAC transgenic constructs; we generate gene targeting animals using ES cell approach for the mouse and CRISPR/Cas9 and integrase approaches for the mouse and the rat; we cryopreserve and recover genetically modified mouse and rat lines.
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Services
- Design and generate gene targeting constructs, including knockin report genes, knockin mutations, conventional/conditional knockouts, and BAC transgenics and constructs for CRISPR/cas9 targeting
- ES cell targeting
- Generation of chimeras through ES cell/embryo aggregation
- DNA pronuclear microinjection and CRISPR/cas9 microinjection
- Establish new ES cell lines from blastocysts
- Mouse Embryo/sperm Cryopreservation and IVF to recover the lines
- Developing new technologies
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