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Gene Targeting and Transgenics

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More in this Support Team
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What We Do

Our Gene Targeting & Transgenic Facility makes sophisticated gene targeting a turnkey service at a low cost. We develop and experiment with new technologies to support research needs. We design and produce gene targeting, CRISPR/Cas9 and BAC transgenic constructs; we generate gene targeting animals using ES cell approach for the mouse and CRISPR/Cas9 and integrase approaches for the mouse and the rat; we cryopreserve and recover genetically modified mouse and rat lines.


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  • Design and generate gene targeting constructs, including knockin report genes, knockin mutations, conventional/conditional knockouts, and BAC transgenics and constructs for CRISPR/cas9 targeting
  • ES cell targeting
  • Generation of chimeras through ES cell/embryo aggregation
  • DNA pronuclear microinjection and CRISPR/cas9 microinjection
  • Establish new ES cell lines from blastocysts
  • Mouse Embryo/sperm Cryopreservation and IVF to recover the lines
  • Developing new technologies
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