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What We Do
The Anatomy and Histology facility turns tissues and whole organs into sections that are suitable for microscope examination or physiological experiments. Think of the anatomy and histology facility as an extension of scientists’ own labs. Whether researchers use the facility’s equipment on their own or utilize the experience of our on-site staff, they have the ability to get more science done effectively and efficiently.
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Scientists have access to 1,000 square feet of workspace for tissue fixation, sectioning, immuno-labeling, and staining of a variety of mouse brains and other cells and tissues. They can tap into staff skilled in antibody-labeling methods and preparing cultured brain slices for physiology experiments. Or they can access a full range of equipment, including two chemical fume hoods for fixatives, a cell culture hood, a clean box, and four CO2 incubators for preparing primary cultures.
Equipment
- Cryostats and vibratomes. Investigators can use two Leica 3050S cryostats, five Leica vibratomes, and a CryoJane tape transfer system.
- Tissue culture hoods. Researchers can use an online scheduler to book culture hoods.
- Microscopes. Examine primary cultures with a Zeiss inverted fluorescence microscope or inspect GFP and antibody labeling in sections with an Olympus MVX10, a fluorescence dissecting microscope.
Resources
- Anesthesia and perfusion-fixation of mice and rats
- Embedding in OTC and agarose
- 5 µm-thick to 50 µm-thick sections prepared on Leica cryostat or vibratome
- Antibody labeling and histochemical staining (Nissl, H&E, special stains)
- Primary cultures of hippocampal neurons
- Preparation of brain slice cultures for physiological recording
- In-situ hybridization
- Training on vibratomes, cryostats, and more
- Imaging
- Expansion microscopy sample preparation and imaging
- Contact us about working with large sample sets
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