What We Do
The Electron Microscopy Shared Resource (EMSR) enables biological research at micro- and nano-scales and provides researchers a nexus for multidisciplinary collaboration in high-resolution imaging. We offer state-of-the-art instrumentation, services, and training in sample preparation, transmission electron microscopy and image analysis. One main function of the facility is to prepare samples for room temperature TEM imaging or volume SEM imaging, using conventional wet chemistry, microwave techniques and high-pressure freezing/freeze substitution. Our skilled staff will assist researchers and train them in the science and practice of electron microscopy as well.
- FEI Tecnai Spirit BioTWIN trasmission electron microscope with LaB6 gun and Gatan Oneview camera
- Zeiss Xradia 510 Versa for submicron X-ray imaging
- Delphi integrated scanning electron microscope and fluorescence microscope
- Wohlwend HPF Compact 03 high-pressure freezer
- Leica EM ICE high pressure freezer
- Leica EM AFS2 automatic freeze-substitution system for processing samples prepared by high-pressure freezing or progressive lowering of temperature (PLT) methods
- Built-in UV lamp for UV-catalyzed resin polymerization at low temperature
- Leica UC7 and UC6 ultramicrotomes for room temperature and cryo semi-thin and ultra-thin sectioning. Each microtome is positioned in a separate room with adequate bench space and ancillary specimen preparation equipment so as to be self-contained sectioning areas
- EMS 100 Glow Discharge unit for making support films on grids hydrophilic
- Pelco BioWave Pro microwave oven with ColdSpot, SteadyTemp, and vacuum chamber options for maximum microwave sample preparation flexibility
- Tousimis Autosamdri-931 critical point dryer
- Leica EM ACE600 sputter coater
- Leica VT1000S Vibrotome
- Sample fixation and processing
- Sample staining and embedding for TEM or volume SEM
- Ultrathin sectioning for TEM
- TEM imaging
- Immuno EM
- Micro CT scan
- High pressure freezing for cryo light microscopy, cryo EM or freeze substitution
- Development of protocols for special research needs
- The EMSR facility mainly provides services to Janelia users. Certain services might be provided to HHMI labs outside of Janelia. for inquiry please contact facility director Zhiheng Yu.
Dr. Wei-Ping Li completed her Ph.D. in cell biology with Jürgen Roth, the “immunogold master” who pioneered the use of immunogold EM on thin sections (Sedwick 2013). Following in the footsteps of her mentor, Dr. Li is Janelia’s immunogold expert, developing protocols that enable researchers to analyze connectivity between identified neurons at the EM level.
Double immunogold labeling in mouse brain is used to distinguish different cell types. Protocol was optimized to preserve antigenicity and synaptic ultrastructure (arrows).
Neuron expressing a fluorescent protein engineered by the Looger Lab was reconstructed from immunogold labeled serial sections of the mouse brain. Synapses (red arrows) are readily identifiable. Figure is modified from Viswanathan et al. (in press).