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Electron Microscopy

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Support Teams / Electron Microscopy
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What We Do

The Electron Microscopy Facility enables biological research at micro- and nano-scales and provides Janelia researchers a nexus for multidisciplinary collaboration in high-resolution imaging. We offer state-of-the-art instrumentation, services, and training in sample preparation, transmission  electron microscopy and image analysis.  With 5,000-square-feet of space, we’re set up to prepare samples using conventional wet chemistry, microwave techniques and high-pressure freezing/freeze substitution. Our skilled staff will assist researchers and train them in the science and practice of electron microscopy as well.

 

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Equipment

 

  • FEI Tecnai Spirit BioTWIN trasmission electron microscope with LaB6 gun and Gatan Oneview camera
  • Zeiss Xradia 510 Versa for submicron X-ray imaging
  • Delphi integrated scanning electron microscope and fluorescence microscope
  • Wohlwend HPF Compact 03 high-pressure freezer
  • Leica EM ICE high pressure freezer
  • Leica EM AFS2 automatic freeze-substitution system for processing samples prepared by high-pressure freezing or progressive lowering of temperature (PLT) methods
  • Built-in UV lamp for UV-catalyzed resin polymerization at low temperature
  • Leica UC7 and UC6 ultramicrotomes for room temperature and cryo semi-thin and ultra-thin sectioning. Each microtome is positioned in a separate room with adequate bench space and ancillary specimen preparation equipment so as to be self-contained sectioning areas
  • EMS 100 Glow Discharge unit for making support films on grids hydrophilic
  • Pelco BioWave Pro microwave oven with ColdSpot, SteadyTemp, and vacuum chamber options for maximum microwave sample preparation flexibility
  • Tousimis Autosamdri-931 critical point dryer
  • Leica EM ACE600 sputter coater
  • Leica VT1000S Vibrotome

Services

  • Operation of microscopes and acquisition of images
  • Sample fixation and processing
  • Sample sectioning and staining for TEM
  • Development of protocols for special research needs
  • Training

 

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