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Equipment
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We have a full complement of state-of-the-art microscopes and multiple workstations dedicated to image processing. Our imaging capabilities support most experiments involving, but not limited to, fixed and live specimens, cultured cells, tissue slices, whole mouse brain, zebrafish and drosophila embryos, etc.

Zeiss LSM 880 with Airyscan (inverted confocal)

   
  • Inverted laser scanning confocal microscope equipped with two multi-Alkali PMTs and 32-channel spectral GaAsP PMTs (up to 13 fps)
  • Light sources: 405/458/488/514/561/594/633 nm laser lines, and X-Cite 120PC Series illuminator
  • Airyscan super-resolution capabilities:
    • 2x better resolution in all three dimensions
    • Improved sensitivity with 4-8x higher SNR
    • More gentle excitation (less photobleaching and phototoxicity)
  • Spectral unmixing capabilities:
    • Acquire 5-8 dyes in parallel with spectral imaging
    • Linear unmixing post-acquisition segregates mixed fluorescence signals from dyes and cellular autofluorescence
  • Airyscan FAST with Z-piezo control for imaging at 4x speed and improved sensitivity
  • Live cell imaging with temperature and CO2 control
  • Experiment designer for advanced acquisitions
  • Additional features include tiling, photomanipulation, definite focus, etc.

Zeiss LSM 880 NLO (upright confocal)

  • Upright confocal and two-photon (2P) laser scanning microscope equipped with two multi-Alkali PMTs, 32-channel spectral GaAsP PMTs (up to 13 fps), and a BiG-2 non-descanned GaAsP PMT
  • Light sources: 405/458/488/514/561/594/633, adjustable 690-1064 nm (Chameleon) laser lines, and HXP 120 illuminator
  • Long working distance, water-dipping objectives
  • Spectral unmixing capabilities:
    • Acquire 5-8 dyes in parallel with spectral imaging
    • Linear unmixing post-acquisition segregates mixed fluorescence signals from dyes and cellular autofluorescence
  • Experiment designer for advanced acquisitions
  • Additional features include tiling, photomanipulation, etc.

Zeiss LSM 980 (inverted confocal)

  • Inverted laser scanning confocal microscope equipped with two multi-Alkali PMTs, 4-channel spectral GaAsP PMTs (up to 13 fps), and AxioCam 506 (monochrome CCD)​
  • Light sources: 405/445/488/514/561/594/639 nm laser lines, and Colibri 5 multi-LED illumination system (385/475/555/630 nm)
  • Experiment designer for advanced acquisitions
  • Additional features include advanced tiling module, AI Sample Finder, Definite Focus 3, etc.

Zeiss Axio Imager.Z1 with ApoTome.2 (upright widefield)

  • Upright widefield microscope with apotome attachment equipped with AxioCam MRc (color) and MRm (monochrome CCD) cameras for high speed (up to 48 fps)
  • Light sources: Colibri.2 multi-LED illumination system (365/470/590 nm) and HXP 120 illuminator with multiple filter sets
  • Long working distance, water-dipping objectives
  • ApoTome.2 high-resolution capabilities:
    • Structured illumination to achieve thin optical sectioning and diffraction-limited images with high SNR
    • ​Tailored for cell cultures, tissue sections of variable thickness, and low-scattering samples
    • 3D reconstructions
  • Additional features include advanced tiling module, dark-field illumination, etc.

Zeiss Lightsheet Z.1 (LSFM)

  • Dual-side illumination light sheet fluorescence microscope (LSFM) equipped with two liquid cooled pco.edge sCMOS cameras for high sensitivity and speed (up to 100 fps)
  • Light sources: 405/445/488/514/561/638 nm laser lines
  • 3D acquisition capabilities:
    • Thin optical sectioning (light sheet thickness ranges from 2-14 µm) with high SNR
    • High-speed imaging with very low phototoxicity and photobleaching
    • Multiple objectives (including long working distance, water-dipping and 20x CLARITY objectives) for aqueous or cleared samples
    • Tailored for zebrafish and drosophila embryos, whole mouse brain, expanded samples, and transparent specimens (up to 5 mm)
  • Live cell imaging with temperature and CO2 control
  • Additional features include pivot scanning, tiling, multiview (360°) imaging, etc.

Custom-built Lattice Light-sheet (LLSM)

  • Two custom-built lattice light-sheet microscopes (LLSM) each equipped with two Hamamatsu ORCA-Flash4.0 V3 sCMOS cameras for high sensitivity and speed (up to 100 fps)
  • Light sources: 405/488/561/642 nm laser lines
  • 3D acquisition capabilities:
    • Thin optical sectioning (light sheet thickness ranges from 400-800 nm) with high SNR and optimal resolution of 230 nm (lateral) and 370 nm (axial)
    • High-speed imaging with very low phototoxicity and photobleaching
    • Long working distance, water-dipping objective for aqueous samples
    • Tailored for zebrafish and drosophila embryos, expanded samples (ExLLSM), and transparent specimens (up to 4 mm)
  • Live cell imaging with temperature control
  • Additional features include advanced tiling etc.

Multimodal Optical Scope with Adaptive Imaging Correction (MOSAIC) - UNDER CONSTRUCTION

  • This instrument combines several imaging modalities for visualizing and quantifying complex biological systems in context with adaptive optics (AO) correction and is equipped with two Hamamatsu ORCA-Flash4.0 V3 sCMOS cameras for high sensitivity and speed (up to 100 fps)
  • Light sources: 405/488/514/560/607/642 and adjustable 680-1080 nm (Chameleon) laser lines
  • MOSAIC will offer multiplexed capabilities combined with AO: LLSM with AO (available), ExLLSM (available), 3D SIM, Multifocal image scanning/pixel reassignment, 2P point scanning, 2P Bessel fast functional imaging, 2P Bessel LSFM, 2P Bessel point scanning, Phase imaging, Patterned photostimulation, and 3D single molecule localization
  • 3D acquisition capabilities:
    • Thin optical sectioning (light sheet thickness ranges from 400-800 nm) with high SNR and optimal resolution of 230 nm (lateral) and 370 nm (axial) as well as extended light sheet imaging tile size (220 µm)
    • High-speed imaging with very low phototoxicity and photobleaching
    • Multiple long working distance, water-dipping objectives
    • Simultaneous two-color or sequential multi-color illumination for all imaging modes
    • AO correction (wavefront sensing) along both excitation and emission light paths for all imaging modes
  • Live cell imaging with temperature and CO2 control
  • Additional features include advanced tiling, photomanipulation, etc.

Nikon CSU-W1 SoRa (inverted spinning disk confocal / TIRF)

  • Inverted spinning disk confocal microscope equipped with one Photometrics Prime 95B 22mm and two Hamamatsu ORCA-Fusion sCMOS cameras for high sensitivity and speed (up to 100 fps)
  • Light sources: 405/445/488/514/561/594/640 and 488/561/640 and 405/473 nm laser lines for confocal microscopy, TIRF, and galvo-based photomanipulation (PM), respectively, as well as Lumencor Spectra-III LED light engines for DMD-based PM and epifluorescence
  • System offers multiplexed capabilities including spinning disk confocal microscopy (50 µm-pinholed disk and SoRa disk mode for 2x better resolution in all three dimensions), TIRF, and large FOV epifluorescence
  • Fast Z-piezo stage
  • Multiple high-performance objectives including SR, TIRF, and silicone immersion lenses optimized for live cell imaging
  • Live cell imaging with temperature, CO2, and hypoxia/hyperoxia (O2) control
  • JOBS module for advanced acquisitions using High Content Analysis with heat maps representation:
    • Create any custom acquisition
    • Automated screening of multi-well plates
    • Intelligent acquisition capable of modifying settings based on real-time image analysis and feedback
  • Additional features include advanced tiling, perfect focus system (PFS), Denoise.ai, water immersion dispenser, etc.

Nikon Eclipse Ti (inverted widefield)

  • Inverted widefield microscope equipped with an Andor Zyla sCMOS camera for high sensitivity and speed (up to 50 fps)
  • Light sources: Lumencor Spectra X LED light engine (V/B/C/G-Y/R/NIR) with multiple filter sets
  • Live cell imaging with temperature and CO2 control
  • JOBS module for advanced acquisitions using High Content Analysis with heat maps representation:
    • Create any custom acquisition
    • Automated screening of multi-well plates
    • Intelligent acquisition capable of modifying settings based on real-time image analysis and feedback
  • Additional features include advanced tiling, PFS, Denoise.ai, etc.

TissueGnostics TissueFAXS 200 (widefield and confocal slide scanner)

  • Combination widefield and spinning disk confocal microscope operated as a whole slide digital scanner equipped with an Andor Zyla sCMOS camera for high sensitivity and speed
  • Light sources: Lumencor Celesta (405/446/477/520/546/638/749 nm laser lines) and two Lumencor Spectra X LED light engines (V/B/C/G-Y/R/NIR) with multiple filter sets
  • High-throughput capabilities including an autoloader cassette that holds up to 200 slides for unsupervised workflow
    • Faster acquisitions are possible by only imaging the tissue sections ("automated" detection)
  • Tailored for multi-color slide scanning, tissue cytometry, 5 µm optical sectioning for samples up to 50 µm-thick, and brightfield imaging of chromogenic stained samples
  • Additional features include digital stitching of whole slides, tools for cytoplasmic membrane or nuclear measurements, advanced image analysis software, etc.
    • Strataquest: Image visualization and analysis software for automated detection/cytometry and context-based quantification tasks across entire slides

3DHISTECH Pannoramic 250 Flash (widefield slide scanner)

  • Widefield microscope operated as a whole slide digital scanner equipped with a pco.edge sCMOS camera
  • Light sources: Lumencor Spectra X LED light engine (V/B/C/G-Y/R/NIR) with multiple filter sets
  • High-throughput capabilities including an autoloader cassette that holds up to 250 slides for unsupervised workflow
  • Tailored for multi-color slide scanning, tissue cytometry, and brightfield imaging of chromogenic stained samples
  • Additional features include digital stitching of whole slides, tools for cytoplasmic membrane or nuclear measurements, CaseCenter management system, etc.

Olympus MVX10 (upright macroscope)

  • Two upright macro zoom microscopes ("macroscopes") equipped with color CCD cameras
  • Light sources: Lumencor SOLA LED light engine with multiple filter sets (DAPI/ECFP/GFP/EYFP/Cy3/mRFP/mCherry/Cy5)
  • Low and high (NA=0.5) magnification objectives for 50x zoom range without major changes in brightness
  • ​Tailored for dissection, long-term observation of whole organisms, and on-the-fly fluorescence imaging​

Olympus BX51 (upright compound)

  • Upright compound microscope equipped with a 12-bit Olympus DP71 color camera
  • 12V/100W halogen illumination source with multiple light filters and objectives including 100x/1.4 oil
  • ​Tailored for color histology/pathology samples and on-the-fly transmitted light viewing

Leica VT1000S (vibratome)

  • Two vibrating blade microtomes ("vibratomes") for high-quality sectioning
  • Section thickness ranges from 100-300 µm for live tissue and from 10-20 µm for fixed tissue
  • Tailored for neurophysiology, neuropathology, and experimental pathology applications