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Multiphoton Microscopy in the Biomedical Sciences XXMultiphoton Microscopy in the Biomedical Sciences XX. 2020 Feb 14;. doi: 10.1117/12.2543232
Multiplexed 3-photon microscopy for functional connectomics of mammalian brains.
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Abstract
3-photon excitation enables in vivo fluorescence microscopy deep in densely labeled and highly scattering samples, while maintaining high resolution and contrast. We designed and characterized a dual-plane 3-photon microscope with temporal multiplexing and remote focusing, and performed simultaneous in vivo calcium imaging of two planes deep in the cortex of a transgenic mouse expressing GCaMP6s in nearly all excitatory neurons.
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Janelia Authors
Dmitri Tsyboulski
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