iAchSnFR: Acetylcholine Sensor

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iAchSnFR: Acetylcholine Sensor

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Genetically Encoded Acetylcholine Sensor

Overview:

A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms, and flies

Acetylcholine receptors are among the most diverse neurotransmitter receptor families
Large fluorescence changes (up to 12-fold)
Millisecond-level Rapid rise and decay kinetics
Insensitivity to most cholinergic drugs
Available in green (default, GFP-based) and yellow (Venus) versions
Optional red fluorescent protein tag (mRuby2) for normalization

Figure 1. Representative images of HEK293 cells and hippocampal neuron cultures in buffer alone, with 100 µM acetylcholine, and transformation of ΔF/F as a heat map. (Borden et al.)

Publication:
Borden, PM et al. bioRxiv 2020-02-07-939504; https://doi.org/10.1101/2020.02.07.939504
Materials and IP including US Patent 11,698,374 are available for license-

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