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The JFX™ Dyes

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The JFX™ Dyes

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The JFX™ Dyes: Harnessing the Power of Deuterium to Improve Brightness and Photostability

Overview

The JFX dye platform enhances the performance of Janelia Fluor® dyes by introducing deuterium at key positions, boosting brightness and extending photostability without altering their spectral signatures or cell permeability.

Key Advantages

  • Higher Quantum Yield and Brightness: Deuterated dyes outperform their hydrogen-containing analogs, delivering brighter fluorescence signals.
  • Superior Photostability: Slower photobleaching allows longer imaging experiments and improved data quality.
  • Preserved Excitation/Emission Wavelengths: Retains 560 nm and 640 nm excitation windows for easy integration into existing imaging setups.
  • Cell-Permeable and Versatile: Compatible with HaloTag®, SNAP-tag®, and standard chemical conjugation strategies.

Spotlight Dyes

  • JFX554: Bright, deuterated green-excited dye for 560 nm excitation.
  • JFX650: Highest-performance far-red dye for demanding super-resolution and SPT experiments.

Applications

  • In vitro single-molecule imaging and live-cell single-particle tracking (SPT)
  • Super-resolution imaging (SMLM, SIM, STED, MINFLUX)
  • Long-duration confocal and two-photon fluorescence microscopy
  • Analytical and diagnostic labeling technologies

Reference: JACS Au 2021, 1, 690–696 https://doi.org/10.1021/jacsau.1c00006

IP Coverage: U.S. Patent Nos. 11,091,643, 11,787,946, and application 20240052169

Janelia Tech ID: 2017-043

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Bright JFX dyes. Credit: Jon Grimm/Janelia Research Campus
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Published Information

Lavis Lab
Exploiting Isotope Effects to Improve Fluorophores
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Janelia Collaborators

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Lavis Lab
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Contact

Michael Perham
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