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GENIE Reagents

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GENIE / GENIE Reagents
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GENIE Reagents
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jRCaMP1 calcium indicators

​Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

CMV-NES-jRCaMP1a

red, improved SNR, slower kinetics, bright

61562

Dana et al., 2016

CMV-NES-jRCaMP1b

red, improved SNR, improved dynamic range, bright

63136

Dana et al., 2016

 

Viruses available from University of Pennsylvania Viral Vector Core:

Name Penn Catalog Number Reference

AAV1.Syn.NES.jRCaMP1a.WPRE.SV40

AV-1-PV3855

Dana et al., 2016

AAV1.CAG.Flex.NES.jRCaMP1a.WPRE.SV40

AV-1-PV3853

Dana et al., 2016

AAV1.Syn.Flex.NES.jRCaMP1a.WPRE.SV40

AV-1-PV3854

Dana et al., 2016

AAV1.Syn.NES.jRCaMP1b.WPRE.SV40

AV-1-PV3852

Dana et al., 2016

AAV1.CAG.Flex.NES.jRCaMP1b.WPRE.SV40

not yet available

Dana et al., 2016

AAV1.Syn.Flex.NES.jRCaMP1b.WPRE.SV40

not yet available

Dana et al., 2016

 

Flies available from Bloomington Stock Center:

Line name Insertion Site Bloomington Stock Number Reference Chr
PBac{w[+mC]=20XUAS-IVS-NES-jRCaMP1a-p10} VK00005 63792 Dana et al., 2016 3
PBac{w[+mC]=20XUAS-IVS-NES-jRCaMP1b-p10} VK00005 63793 Dana et al., 2016 3

 

jRGECO1 calcium indicators

Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

CMV-NES-jRGECO1a

red, improved SNR

61563

Dana et al., 2016

 

Viruses available from University of Pennsylvania Viral Vector Core:

Name Penn Catalog Number Reference

AAV1.Syn.NES.jRGECO1a.WPRE.SV40

AV-1-PV3849

Dana et al., 2016

AAV1.CAG.Flex.NES.jRGECO1a.WPRE.SV40

not yet available

Dana et al., 2016

AAV1.Syn.Flex.NES.jRGECO1a.WPRE.SV40

AV-1-PV3848

Dana et al., 2016

AAV1.Syn.NES.jRGECO1b.WPRE.SV40

AV-1-PV3846

Dana et al., 2016

AAV1.CAG.Flex.NES.jRGECO1b.WPRE.SV40

not yet available

Dana et al., 2016

AAV1.Syn.Flex.NES.jRGECO1b.WPRE.SV40

not yet available

Dana et al., 2016

 

Flies available from Bloomington Stock Center:

Line name Insertion Site Bloomington Stock Number Reference Chr
PBac{w[+mC]=20XUAS-IVS-NES-jRGECO1a-p10} VK00005 63794 Dana et al., 2016 3
PBac{w[+mC]=20XUAS-IVS-NES-jRGECO1b-p10} VK00005 63795 Dana et al., 2016 3

 

GCaMP6 calcium indicators

Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

CMV-GCaMP6s

improved SNR, slower kinetics

40753

Chen et al., 2013

CMV-GCaMP6m

improved SNR, intermediate kinetics

40754

Chen et al., 2013

CMV-GCaMP6f

improved SNR, faster kinetics

40755

Chen et al., 2013

 

Viruses available from University of Pennsylvania Viral Vector Core:

Name Penn Catalog Number Penn Map and Sequence Number Reference

AAV1.Syn.GCaMP6f.WPRE.SV40

AV-1-PV2822

Penn Vector P2822

Chen et al., 2013

AAV1.Syn.GCaMP6s.WPRE.SV40

AV-1-PV2824

Penn Vector P2824

Chen et al., 2013

AAV1.Syn.GCaMP6m.WPRE.SV40

AV-1-PV2823

Penn Vector P2823

Chen et al., 2013

AAV5.Syn.GCaMP6m.WPRE.SV40

AV-5-PV2823

Penn Vector P2823

Chen et al., 2013

AAV5.Syn.GCaMP6f.WPRE.SV40

AV-5-PV2822

Penn Vector P2822

Chen et al., 2013

AAV5.Syn.GCaMP6s.WPRE.SV40

AV-5-PV2824

Penn Vector P2824

Chen et al., 2013

AAV9.Syn.GCaMP6f.WPRE.SV40

AV-9-PV2822

Penn Vector P2822

Chen et al., 2013

AAV9.Syn.GCaMP6m.WPRE.SV40

AV-9-PV2823

Penn Vector P2823

Chen et al., 2013

AAV9.Syn.GCaMP6s.WPRE.SV40

AV-9-PV2824

Penn Vector P2824

Chen et al., 2013

AAV1.CAG.GCaMP6s.WPRE.SV40

AV-1-PV2833

Penn Vector P2833

Chen et al., 2013

AAV5.CAG.GCaMP6s.WPRE.SV40

AV-5-PV2833

Penn Vector P2833

Chen et al., 2013

AAV9.CAG.GCaMP6s.WPRE.SV40

AV-9-PV2833

Penn Vector P2833

Chen et al., 2013

AAV1.CAG.Flex.GCaMP6f.WPRE.SV40

AV-1-PV2816

Penn Vector 2816

Chen et al., 2013

AAV1.CAG.Flex.GCaMP6m.WPRE.SV40

AV-1-PV2817

Penn Vector 2817

Chen et al., 2013

AAV1.CAG.Flex.GCaMP6s.WPRE.SV40

AV-1-PV2818

Penn Vector 2818

Chen et al., 2013

AAV1.Syn.Flex.GCaMP6f.WPRE.SV40

AV-1-PV2819

Penn Vector 2819

Chen et al., 2013

AAV1.Syn.Flex.GCaMP6m.WPRE.SV40

AV-1-PV2820

Penn Vector 2820

Chen et al., 2013

AAV1.Syn.Flex.GCaMP6s.WPRE.SV40

AV-1-PV2821

Penn Vector 2821

Chen et al., 2013

AAV5.CAG.Flex.GCaMP6f.WPRE.SV40

AV-5-PV2816

Penn Vector 2816

Chen et al., 2013

AAV5.CAG.Flex.GCaMP6m.WPRE.SV40

AV-5-PV2817

Penn Vector 2817

Chen et al., 2013

AAV5.CAG.Flex.GCaMP6s.WPRE.SV40

AV-5-PV2818

Penn Vector 2818

Chen et al., 2013

AAV5.Syn.Flex.GCaMP6f.WPRE.SV40

AV-5-PV2819

Penn Vector 2819

Chen et al., 2013

AAV5.Syn.Flex.GCaMP6m.WPRE.SV40

AV-5-PV2820

Penn Vector 2820

Chen et al., 2013

AAV5.Syn.Flex.GCaMP6s.WPRE.SV40

AV-5-PV2821

Penn Vector 2821

Chen et al., 2013

AAV9.CAG.Flex.GCaMP6f.WPRE.SV40

AV-9-PV2816

Penn Vector 2816

Chen et al., 2013

AAV9.CAG.Flex.GCaMP6m.WPRE.SV40

AV-9-PV2817

Penn Vector 2817

Chen et al., 2013

AAV9.CAG.Flex.GCaMP6s.WPRE.SV40

AV-9-PV2818

Penn Vector 2818

Chen et al., 2013

AAV9.Syn.Flex.GCaMP6f.WPRE.SV40

AV-9-PV2819

Penn Vector 2819

Chen et al., 2013

AAV9.Syn.Flex.GCaMP6m.WPRE.SV40

AV-9-PV2820

Penn Vector 2820

Chen et al., 2013

AAV9.Syn.Flex.GCaMP6s.WPRE.SV40

AV-9-PV2821

Penn Vector 2821

Chen et al., 2013

 

 

Mice available from The Jackson Laboratory:

Name Description Jax Stock Number Reference

Thy1-GCaMP6s-WPRE (founder line GP4.3)

improved SNR, slower kinetics, variable neuronal expression pattern

024275

Dana et al., 2015

Thy1-GCaMP6s-WPRE (founder line GP4.6) improved SNR, slower kinetics, variable neuronal expression pattern 028278 Dana et al., 2015

Thy1-GCaMP6s-WPRE (founder line GP4.12)

improved SNR, slower kinetics, variable neuronal expression pattern

025776

Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.1) improved SNR, faster kinetics, variable neuronal expression pattern 028279 Dana et al., 2015
Thy1-GCaMP6f-WPRE (founder line GP5.3) improved SNR, faster kinetics, variable neuronal expression pattern 028280 Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.5)

improved SNR, faster kinetics, variable neuronal expression pattern

024276

Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.11)

improved SNR, faster kinetics, variable neuronal expression pattern

024339

Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.12) improved SNR, faster kinetics, variable neuronal expression pattern 028281 Dana et al., 2015

Thy1-GCaMP6f-WPRE (founder line GP5.17)

improved SNR, faster kinetics, variable neuronal expression pattern

025393

Dana et al., 2015

 

 

Flies available from Bloomington Stock Center:

Line name Insertion Site Bloomington Stock Number Reference Chr
P{20XUAS-IVS-GCaMP6s} attP40 42476 Chen et al., 2013 2
P{20XUAS-IVS-GCaMP6m} attP40 42748 Chen et al., 2013 2
P{20XUAS-IVS-GCaMP6f} attP40 42747 Chen et al., 2013 2
PBac{20XUAS-IVS-GCaMP6s} VK00005 42749 Chen et al., 2013 3
PBac{20XUAS-IVS-GCaMP6m} VK00005 42750 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6s-SV40} su(Hw)attP1 44273 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6m-SV40} su(Hw)attP1 44588 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6s-SV40} su(Hw)attP5 44589 Chen et al., 2013 2
P{13XLexAop2-IVS-GCaMP6s-p10} su(Hw)attP1 44274 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6m-p10} su(Hw)attP1 44275 Chen et al., 2013 3
P{13XLexAop2-IVS-GCaMP6s-p10} su(Hw)attP5 44590 Chen et al., 2013 2
P{13XLexAop2-IVS-GCaMP6f-p10} su(Hw)attP5 44277 Chen et al., 2013 2
P{13XLexAop2-IVS-GCaMP6m-p10} VK00005 44276 Chen et al., 2013 3

 

C. elegans distributed upon request (contact Program Scientist):

Name

mec-4::nls-RSET-GCaMP6s:SL2:nls-TagRFP::unc-54utr

GCaMP5 calcium indicators

Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

CMV-GCaMP5G

Green calcium indicator

31788

Akerboom et al., 2012

 

Viruses available from University of Pennsylvania Viral Vector Core:

Name Penn Catalog Number Penn Map and Sequence Number Reference

AAV1.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-1-PV2478

Penn Vector P2478

Akerboom et al., 2013

AAV5.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-5-PV2478

Penn Vector P2478

Akerboom et al., 2013

AAV9.hSynap.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2478

Penn Vector P2478

Akerboom et al., 2013

AAV9.CAG.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2643

Penn Vector P2643

Akerboom et al., 2013

AAV1.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-1-PV2541

Penn Vector P2541

Akerboom et al., 2013

AAV9.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2541

Penn Vector P2541

Akerboom et al., 2013

AAV1.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-1-PV2540

Penn Vector 2540

Akerboom et al., 2013

AAV5.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-5-PV2540

Penn Vector P2540

Akerboom et al., 2013

AAV9.hSynap.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40

AV-9-PV2540

Penn Vector P2540

Akerboom et al., 2013

 

Flies available from Bloomington Stock Center:

Name Insertion Site Bloomington Stock Number Reference Chr
P{20XUAS-IVS-GCaMP5G} attP40 42037 Akerboom et al., 2012  2
P{20XUAS-IVS-GCaMP5G} VK00005 42038 Akerboom et al., 2012  3

GCaMP3 calcium indicators

Plasmids available from Addgene.org:

Name Description Addgene Plasmid ID Reference

​CMV-GCaMP3

Green calcium indicator

22692

Tian et al., 2009

 

Viruses available from University of Pennsylvania Viral Vector Core:

Name Penn Catalog Number Penn Map and Sequence Number Reference
AAV1.hSynap.GCaMP3.WPRE.SV40

AV-1-PV1627

Penn Vector P1627

Tian et al., 2013

AAV5.hSynap.GCaMP3.WPRE.SV40

AV-5-PV1627

Penn Vector P1627

Tian et al., 2013

AAV9.hSynap.GCaMP3.WPRE.SV40

AV-9-PV1627

Penn Vector P1627

Tian et al., 2013

AAV1.hSynap.Flex.GCaMP3.WPRE.SV40

AV-1-PV1921

Penn Vector P1921

Tian et al., 2013

AAV5.hSynap.Flex.GCaMP3.WPRE.SV40

AV-5-PV1921

Penn Vector P1921

Tian et al., 2013

AAV9.hSynap.Flex.GCaMP3.WPRE.SV40

AV-9-PV1921

Penn Vector P1921

Tian et al., 2013

 

Mice available from The Jackson Laboratory:

Name Jax Stock Number Reference

Rosa26-CAG-lox-stop-lox-GCaMP3-WPRE

014538

Zariwala et al., 2010

 

Flies available from Bloomington Stock Center:

Name Insertion Site Bloomington Stock Number Reference Chr

P{UAS-GCaMP3.T}

attP40

32116

Tian et al., 2009

 2

P{20XUAS-GCaMP3} su(Hw)attP8 32234 Tian et al., 2009  1
P{20XUAS-GCaMP3} attP18 32235 Tian et al., 2009  1
P{20XUAS-GCaMP3} attP2 32236 Tian et al., 2009  3
PBac{20XUAS-GCaMP3} VK00005 32237 Tian et al., 2009  3

 

 

Data and reagent release policy

We distribute reagents within nine months after initial discovery, often long before publication. 

The data characterizing reagents is published and/or made available on this website (see Open Science). DNA constructs will be deposited at Addgene; viruses at the University of Pennsylvania Vector Core; mice at The Jackson Laboratory; Drosophila at the Bloomington Drosophila Stock Center. C. elegans lines will be distributed upon request.

Reagents can also be made available to collaborators who agree to testing sensor variants using rigorous procedures with jointly agreed upon standards of merit.