A genetically encoded fluorescent biosensor for extracellular L-lactate.

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Nature Communications. 2021 Dec 06;12(1):7058. doi: 10.1038/s41467-021-27332-2
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A genetically encoded fluorescent biosensor for extracellular L-lactate.
Nasu Y, Murphy-Royal C, Wen Y, Haidey JN, Molina RS, Aggarwal Abhi, Zhang S, Kamijo Y, Paquet M, Podgorski K, Drobizhev M, Bains JS, Lemieux MJ, Gordon GR, Campbell RE
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Abstract

L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.

PMID: 34873165 [PubMed - indexed for MEDLINE]

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Janelia Authors

Abhi Aggarwal
Kaspar Podgorski
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